Mousa Ali Omran, Al Hussaini Ali Hussien Abass, Hussein Hashim Mueen
Department of Periodontics, College of Dentistry, University of Baghdad, Baghdad, Iraq.
Department of Oral and Maxillofacial Surgery, College of Dentistry, University of Baghdad, Baghdad, Iraq.
Eur J Dent. 2024 Oct;18(4):1142-1148. doi: 10.1055/s-0044-1782211. Epub 2024 May 14.
Neutrophils own an arsenal of dischargeable chemicals that enable them to handle bacterial challenges, manipulating innate immune response and actual participation in acquired immunity. The reactive oxygen species (ROS) are one of the most important chemicals that neutrophils discharge to eradicate pathogens. Despite their beneficial role, the ROS were strongly correlated to periodontal tissue destruction. Lowdensity neutrophils (LDN) have been recognized for producing enhanced quantities of ROS. However, the potential role of ROS produced by LDN in periodontitis is unknown. The aim of the study was to investigate the impact of ROS produced by LDN in periodontal diseases.
Venous blood and periodontal parameters were obtained from 100 systemically healthy subjects divided into 40 participants with healthy periodontium in the control group and 60 with unstable periodontitis in the study group. Flow cytometry was used to measure the production of ROS by LDN in both groups.
The data were analyzed for normal distribution using the Shapiro-Wilk test at < 0.05, Spearman's correlations, and Mann-Whitney U test. Statistical analysis was performed in SPSS v25.
No difference between the groups had been obtained in ROS production by LDN. However, a significant positive correlation existed between ROS and clinical attachment loss in periodontitis.
LDN exhibits the same ROS generation capacity in the control and periodontitis groups.
中性粒细胞拥有一系列可释放的化学物质，使其能够应对细菌挑战，调控先天免疫反应并实际参与获得性免疫。活性氧（ROS）是中性粒细胞释放以根除病原体的最重要化学物质之一。尽管它们具有有益作用，但ROS与牙周组织破坏密切相关。低密度中性粒细胞（LDN）已被认为会产生更多的ROS。然而，LDN产生的ROS在牙周炎中的潜在作用尚不清楚。本研究的目的是调查LDN产生的ROS对牙周疾病的影响。
从100名全身健康的受试者获取静脉血和牙周参数，将其分为对照组40名牙周健康参与者和研究组60名患有不稳定牙周炎的参与者。采用流式细胞术测量两组中LDN产生ROS的情况。
使用Shapiro-Wilk检验（P<0.05）、Spearman相关性分析和Mann-Whitney U检验对数据进行正态分布分析。在SPSS v25中进行统计分析。
两组中LDN产生ROS的情况无差异。然而，在牙周炎中ROS与临床附着丧失之间存在显著正相关。
在对照组和牙周炎组中，LDN表现出相同的ROS生成能力。
