Pan Hua, Peng Hongwei, Dai Yun, Han Bin, Yang Guangming, Jiang Nian, Zhou Ping
Liyang People's Hospital, Liyang, China.
Faculty of Medicine, University of Tsukuba, Tsukuba, Japan.
J Oncol. 2023 Feb 8;2023:1244067. doi: 10.1155/2023/1244067. eCollection 2023.
Investigate the influence of miR-107 on breast cancer cell growth and death through the PTEN/AKT signaling pathway.
As study subjects, the human breast cancer cell line MCF-7 and the normal breast cell line Hs 578Bst were chosen, and MCF-7 cells were, respectively, transfected with control miRNA and miR-107 inhibitor. CCK-8, flow cytometry, scratch assay, and Transwell assay were used to analyze the proliferation, apoptosis, and invasion, and in order to identify the proteins associated with apoptosis in each of the three categories, we used western blot analysis. Bcl-2, cleaved caspase-3, and cleaved caspase-9 expression, as well as PTEN/AKT signaling pathway-associated protein expression, are correlated.
The expression of miR-107 in MCF-7 cells was significantly greater than that in Hs 578Bst cells, with a < 0.05 difference; compared to the blank and miRNA control groups, the miR-107 inhibitor group had a < 0.05 difference. < 0.05 showed a decrease in proliferation (42.52) but no difference in proliferation between the blank and miRNA control groups ( > 0.05); the miR-107 inhibitor group had higher apoptosis (38.96) with < 0.05 than the blank group (4.85) and the miRNA control group (5.89); there was no difference in apoptosis between the blank and miRNA groups ( > 0.05). There was no significant difference between the blank group and the miRNA control group with > 0.05; compared with the blank group, the miR-107 inhibitor group had a lower expression of Bcl-2 protein (0.18), in addition to the degraded paradigms (0.73) and caspase-9 protein concentrations (0.79), respectively.
The PTEN/AKT signaling pathway may be regulated by miR-107 to limit breast cancer cell growth and increase apoptosis, which suggests that miR-107 may be exploited as a tumor marker for therapeutic therapy.
通过PTEN/AKT信号通路研究miR-107对乳腺癌细胞生长和死亡的影响。
选取人乳腺癌细胞系MCF-7和正常乳腺细胞系Hs 578Bst作为研究对象，分别用对照miRNA和miR-107抑制剂转染MCF-7细胞。采用CCK-8法、流式细胞术、划痕试验和Transwell试验分析细胞增殖、凋亡和侵袭情况，为鉴定这三类细胞中与凋亡相关的蛋白质，我们采用了蛋白质印迹分析。Bcl-2、裂解的caspase-3和裂解的caspase-9表达以及与PTEN/AKT信号通路相关的蛋白质表达具有相关性。
MCF-7细胞中miR-107的表达明显高于Hs 578Bst细胞，差异有统计学意义（P<0.05）；与空白组和miRNA对照组相比，miR-107抑制剂组差异有统计学意义（P<(此处原文有误，推测应该也是0.05)）。P<0.05表明增殖减少（42.52），但空白组和miRNA对照组之间增殖无差异（P>0.05）；miR-107抑制剂组凋亡率较高（38.96），与空白组（4.85）和miRNA对照组（5.89）相比，差异有统计学意义（P<0.05）；空白组和miRNA组之间凋亡无差异（P>0.05）。空白组和miRNA对照组之间差异无统计学意义（P>0.05）；与空白组相比，miR-107抑制剂组Bcl-2蛋白表达较低（0.18），此外，降解模式（0.73）和caspase-9蛋白浓度（0.79）也较低。
PTEN/AKT信号通路可能受miR-107调控，以限制乳腺癌细胞生长并增加凋亡，这表明miR-107可能被用作治疗性治疗的肿瘤标志物。
