Duan Yang, Sun Fuqiang, Li Yueqin, Yang Suyan
Department of Neonatology, Second Hospital of Tianjin Medical University, No. 23 Pingjiang Road, Hexi District, Tianjin 300211, China.
Open Life Sci. 2023 Feb 7;18(1):20220561. doi: 10.1515/biol-2022-0561. eCollection 2023.
Few studies focused on the roles of high glucose combined with high lipid in placental development or fetal growth. This study was designed to investigate the roles of high glucose combined with high lipid in mitochondrial dysfunction of JEG-3 cells. We determined the cellular proliferation and apoptosis, superoxide dismutase (SOD) activity, concentration of malondialdehyde (MDA), and lactic acid dehydrogenase in control group, high glucose group, high lipid group, and high glucose and high lipid group, together with the mitochondrial dysfunction, Nrf2, HO-1, SMAC, and cytochrome (Cyt-) expression. Significant decrease of SOD and significant elevation of MDA was seen in high glucose and high lipid group compared with the other three groups. There was significant decrease in mitochondrial SMAC and Cyt- in high glucose group, high lipid group, and high glucose and high lipid group compared with those of control group. Nrf2 and HO-1 protein expression in high glucose combined with high lipid group showed significant decrease compared with that of high lipid group or high glucose group. We speculated that combination of high glucose and high lipid induced oxidative stress in JEG-3 cells, and Nrf2/ARE pathway may be related to this process.
很少有研究关注高糖合并高脂在胎盘发育或胎儿生长中的作用。本研究旨在探讨高糖合并高脂在JEG - 3细胞线粒体功能障碍中的作用。我们测定了对照组、高糖组、高脂组和高糖高脂组的细胞增殖与凋亡、超氧化物歧化酶（SOD）活性、丙二醛（MDA）浓度以及乳酸脱氢酶，并检测了线粒体功能障碍、Nrf2、HO - 1、SMAC和细胞色素（Cyt -）的表达。与其他三组相比，高糖高脂组的SOD显著降低，MDA显著升高。与对照组相比，高糖组、高脂组和高糖高脂组的线粒体SMAC和Cyt -显著降低。与高脂组或高糖组相比，高糖合并高脂组的Nrf2和HO - 1蛋白表达显著降低。我们推测高糖与高脂联合诱导了JEG - 3细胞的氧化应激，且Nrf2/ARE通路可能与此过程有关。
