Maruszewska-Cheruiyot Marta, Machcińska Maja, Kierasińska Magdalena, Donskow-Lysoniewska Katarzyna
Department of Experimental Immunotherapy, Faculty of Medicine, Lazarski University, 02-662 Warsaw, Poland.
Laboratory of Parasitology, General Karol Kaczkowski Military Institute of Hygiene and Epidemiology, 01-163 Warsaw, Poland.
Oncol Lett. 2023 Jan 25;25(3):93. doi: 10.3892/ol.2023.13680. eCollection 2023 Mar.
Dendritic cells (DCs) are crucial in the development of immune responses. DC JAWS II is a murine cell line frequently used in DC studies. These cells are grown in two cell fractions: Adherent and non-adherent. The present study aimed to compare these two fractions in both immature and lipopolysaccharide (LPS)-activated JAWS II cells. The present study analysed the condition, phenotype, antigen uptake capability, signalling properties and the influence on the activity of T cells using flow cytometry, mixed cell reaction and ELISA methods. Adherent immature JAWS II cells exhibited increased endocytosis and decreased activation of the Pi3K signalling pathway. After LPS activation, adherent JAWS II cells exhibited increased expression levels of CD80 and CD86 costimulatory molecules, increased endocytosis and an elevated ability to induce T cell proliferation, compared with non-adherent cells. These results demonstrated that the two fractions of JAWS II adherent and non-adherent cells exhibited different properties and this should be taken into account in the planning of research.
树突状细胞（DCs）在免疫反应的发展中至关重要。DC JAWS II是一种常用于DC研究的小鼠细胞系。这些细胞以两种细胞组分生长：贴壁细胞和非贴壁细胞。本研究旨在比较未成熟和脂多糖（LPS）激活的JAWS II细胞中的这两种组分。本研究使用流式细胞术、混合细胞反应和ELISA方法分析了细胞状态、表型、抗原摄取能力、信号特性以及对T细胞活性的影响。贴壁未成熟JAWS II细胞表现出内吞作用增加以及Pi3K信号通路激活减少。LPS激活后，与非贴壁细胞相比，贴壁JAWS II细胞表现出共刺激分子CD80和CD86表达水平增加、内吞作用增加以及诱导T细胞增殖的能力增强。这些结果表明，JAWS II贴壁细胞和非贴壁细胞的两种组分表现出不同的特性，在研究规划中应考虑到这一点。
