Fang Wangkai, Fan Tingyu, Xu Liangji, Wang Shun, Wang Xingming, Lu Akang, Chen Yongchun
School of Earth and Environment, Anhui University of Science and Technology, Huainan, China.
Anhui Engineering Laboratory for Comprehensive Utilization of Water and Soil Resources and Ecological Protection in Mining Area With High Groundwater Level, Huainan, China.
Front Microbiol. 2023 Feb 3;14:1098236. doi: 10.3389/fmicb.2023.1098236. eCollection 2023.
Coal mining subsidence lakes are classic hydrologic characteristics created by underground coal mining and represent severe anthropogenic disturbances and environmental challenges. However, the assembly mechanisms and diversity of microbial communities shaped by such environments are poorly understood yet. In this study, we explored aquatic bacterial community diversity and ecological assembly processes in subsidence lakes during winter and summer using 16S rRNA gene sequencing. We observed that clear bacterial community structure was driven by seasonality more than by habitat, and the α-diversity and functional diversity of the bacterial community in summer were significantly higher than in winter ( < 0.001). Canonical correspondence analysis indicated that temperature and chlorophyll-a were the most crucial contributing factors influencing the community season variations in subsidence lakes. Specifically, temperature and chlorophyll-a explained 18.26 and 14.69% of the community season variation, respectively. The bacterial community variation was driven by deterministic processes in winter but dominated by stochastic processes in summer. Compared to winter, the network of bacterial communities in summer exhibited a higher average degree, modularity, and keystone taxa (hubs and connectors in a network), thereby forming a highly complex and stable community structure. These results illustrate the clear season heterogeneity of bacterial communities in subsidence lakes and provide new insights into revealing the effects of seasonal succession on microbial assembly processes in coal mining subsidence lake ecosystems.
采煤塌陷湖是地下采煤形成的典型水文特征，代表着严重的人为干扰和环境挑战。然而，对于受此类环境影响形成的微生物群落的组装机制和多样性，我们目前了解甚少。在本研究中，我们利用16S rRNA基因测序技术，探究了塌陷湖冬季和夏季水生细菌群落的多样性及生态组装过程。我们观察到，细菌群落结构的差异主要由季节因素而非栖息地驱动，并且夏季细菌群落的α多样性和功能多样性显著高于冬季（<0.001）。典范对应分析表明，温度和叶绿素a是影响塌陷湖群落季节变化的最关键因素。具体而言，温度和叶绿素a分别解释了群落季节变化的18.26%和14.69%。冬季细菌群落的变化受确定性过程驱动，而夏季则以随机过程为主。与冬季相比，夏季细菌群落网络表现出更高的平均度、模块性和关键类群（网络中的枢纽和连接点），从而形成了高度复杂且稳定的群落结构。这些结果揭示了塌陷湖细菌群落明显的季节异质性，并为揭示季节性演替对采煤塌陷湖生态系统中微生物组装过程的影响提供了新的见解。
