采煤塌陷区土壤微生物群的季节变化及其与环境因子的关系
Seasonal changes of soil microbiota and its association with environmental factors in coal mining subsidence area.
作者信息
Wang Meng, Sun Mengyao, Zhao Yue, Shi Yuying, Sun Shuo, Wang Shen, Zhou Yiping, Chen Lei
机构信息
Technology Innovation Center of Restoration and Reclamation in Mining induced Subsidence Land, Shandong Provincial Lunan Geology and Exploration Institute (Shandong Provincial Bureau of Geology and Mineral Resources, Ministry of Natural Resources of China, No.2 Geological Brigade), Jining, 272000, China.
College of Life Sciences, Qufu Normal University, Qufu, Shandong Province, China.
出版信息
AMB Express. 2023 Dec 20;13(1):147. doi: 10.1186/s13568-023-01653-5.
BACKGROUND
As a special type of wetland, the new wetland in the coal mining subsidence area is highly sensitive to environmental changes. In recent years, more and more attention has been paid to the studies of soil microbial diversity in newly born wetlands in coal mining subsidence areas. However, there are few reports on the seasonal variation of soil microbial diversity and its relationship with soil physical and chemical properties.
METHODS
In this study, 16S rRNA gene sequencing technology was used to analyze the seasonal changes of soil microbial composition and functional diversity in newly formed wetlands in coal mining subsidence areas, and to determine the seasonal changes of soil nutrient elements and physical and chemical properties in coal mining subsidence areas, so as to analyze the correlation between soil microbial diversity and soil nutrient elements and physical and chemical properties in newly formed wetlands in coal mining subsidence areas.
RESULTS
A total of 16,050 OTUs were obtained after sample gene noise reduction. Proteobacteria, Acidobacteriota and Bacteroidota were the highest abundance in the coal mining subsidence area of Jining. The two seasons gathered separately, and temperature (Temp), total phosphorus (TP), available phosphorus (AP), total organic carbon (TOC) and dry matter content (DMC) were the key factors for the seasonal change of soil microbial community in the wetland of the coal mining subsidence area of Jining. The contents of Temp, AP and TP were significantly correlated with the abundance of soil microorganisms in summer subsidence area, while the contents of DMC and TOC were significantly correlated with the abundance of soil microorganisms in winter subsidence area.
CONCLUSION
Soil microbial diversity in coal mining subsidence area was correlated with the seasons. Temp, TP, AP, TOC and DMC were the key factors for the seasonal change of soil microbial community in the wetland of the coal mining subsidence area of Jining.
背景
作为一种特殊类型的湿地，煤矿开采沉陷区新生湿地对环境变化高度敏感。近年来，煤矿开采沉陷区新生湿地土壤微生物多样性的研究受到越来越多的关注。然而，关于土壤微生物多样性的季节变化及其与土壤理化性质关系的报道较少。
方法
本研究采用16S rRNA基因测序技术，分析煤矿开采沉陷区新生湿地土壤微生物组成和功能多样性的季节变化，测定煤矿开采沉陷区土壤养分元素和理化性质的季节变化，以分析煤矿开采沉陷区新生湿地土壤微生物多样性与土壤养分元素和理化性质之间的相关性。
结果
样本基因降噪后共获得16050个操作分类单元（OTUs）。变形菌门、酸杆菌门和拟杆菌门在济宁煤矿开采沉陷区的丰度最高。两个季节分别聚类，温度（Temp）、总磷（TP）、有效磷（AP）、总有机碳（TOC）和干物质含量（DMC）是济宁煤矿开采沉陷区湿地土壤微生物群落季节变化的关键因素。夏季沉陷区Temp、AP和TP含量与土壤微生物丰度显著相关，而冬季沉陷区DMC和TOC含量与土壤微生物丰度显著相关。
结论
煤矿开采沉陷区土壤微生物多样性与季节相关。Temp、TP、AP、TOC和DMC是济宁煤矿开采沉陷区湿地土壤微生物群落季节变化的关键因素。
Zotero 插件