烟草坏死病毒的结晶及初步X射线衍射研究

Crystallization and preliminary X-ray diffraction studies of tobacco necrosis virus.

作者信息

Fukuyama K, Hirota S, Tsukihara T

机构信息

Faculty of Engineering, Tottori University, Japan.

出版信息

J Mol Biol. 1987 Aug 20;196(4):961-2. doi: 10.1016/0022-2836(87)90421-9.

DOI:10.1016/0022-2836(87)90421-9

PMID:3681987

Abstract

Tobacco necrosis virus is a spherical plant virus consisting of 180 copies of coat protein and a single-stranded RNA. The virus has been crystallized in cubic space group P4(2)32 with a = 338 A. The locations and the orientations of the two virus particles in the unit cell have been determined on the basis of the symmetries of both the particle and the crystal. The crystal diffracts X-rays to at least 2.5 A resolution and is quite stable to X-ray beams (1 A = 0.1 nm).

摘要

烟草坏死病毒是一种球形植物病毒，由180个衣壳蛋白拷贝和一条单链RNA组成。该病毒已在空间群为P4(2)32（a = 338 Å）的立方晶体中结晶。基于病毒颗粒和晶体的对称性，已确定了晶胞中两个病毒颗粒的位置和取向。该晶体对X射线的衍射分辨率至少为2.5 Å，并且对X射线束相当稳定（1 Å = 0.1 nm）。