双层球形病毒——水稻矮缩病毒的结晶及X射线初步研究

Crystallization and preliminary X-ray study of a double-shelled spherical virus, rice dwarf virus.

作者信息

Mizuno H, Kano H, Omura T, Koizumi M, Kondoh M, Tsukihara T

机构信息

National Institute of Agrobiological Resources, Ibaraki, Japan.

出版信息

J Mol Biol. 1991 Jun 20;219(4):665-9. doi: 10.1016/0022-2836(91)90663-q.

DOI:10.1016/0022-2836(91)90663-q

PMID:2056533

Abstract

Rice dwarf virus (RDV) is a double-shelled spherical plant virus consisting of 46,000 Mr capsid and 114,000 Mr core proteins and minor structural proteins, and containing 12 genome segments of double-stranded RNA. The virus has been crystallized in the cubic space group I23 with a = 789 A. There are two particles per unit cell, each positioned on a point of 23 symmetry. Packing considerations showed that the diameter of the virus particle is 693 A. The crystals diffract to at least 6.5 A resolution.

摘要

水稻矮缩病毒（RDV）是一种双层壳球形植物病毒，由分子量为46,000的衣壳蛋白、分子量为114,000的核心蛋白和次要结构蛋白组成，并含有12个双链RNA基因组片段。该病毒已在立方空间群I23中结晶，a = 789 Å。每个晶胞中有两个颗粒，每个颗粒位于23对称点上。堆积分析表明病毒颗粒直径为693 Å。这些晶体的衍射分辨率至少为6.5 Å。