Glycoprotein non-metastatic melanoma B interacts with epidermal growth factor receptor to regulate neural stem cell survival and differentiation.

The functional recovery following spinal cord injury (SCI) remains a challenge clinically. Among the proteins interacted with the , during activation is able to promote the proliferation of neural stem cells (NSCs) in the spinal cord. We investigated the roles of and in regulating the survival and differentiation of the NSCs. By overexpression and short-hairpin RNA-mediated knockdown of in the NSCs, promoted cell viability and differentiation by increasing the expressions of βIII tubulin and CNPase (2',3'-cyclic nucleotide 3-phosphodiesterase). Using co-immunoprecipitation, we found that interacted with . Furthermore, had a similar effect as on promoting cell viability and differentiation. Overexpression of reversed the decrease in viability and differentiation caused by the knockdown of , and . Last but not least, we tested the effect of GPNMB and EGFR on several intracellular pathways and found that modulated the phosphorylated (p)-c-Jun N-terminal kinase (JNK)1/2/JNK1/2 ratio and the p-nuclear factor κB (NF-κB p65)/NF-κB p65 ratio. In sum, our findings demonstrate the interaction between and that regulates cell bioprocesses, with the hope to provide a new strategy of SCI therapy.