Department of Clinical Medicine, West Anhui Health Vocational College, Anhui Province, China.
Department of General Surgery, Affiliated Hospital of Wanxi Health Vocational College, Anhui Province, China.
Medicine (Baltimore). 2023 Feb 10;102(6):e32853. doi: 10.1097/MD.0000000000032853.
Circular RNAs (CircRNAs) have been reported to play key roles in the progression of various cancers, including thyroid cancer (TC). Transcription factor 1 (SP1) promotes the development of thyroid cancer. This study aims at investigating the expression level of Circ0005654 in combination with Transcription factor1 (SP1) in patients with TC for diagnostic and therapeutic purposes. A total of 76 patients with thyroid cancer underwent radical surgery. Intraoperatively, thyroid cancer tissues and paired adjacent tissues and the corresponding clinicopathological data were collected. The expression of SP1 and β-catenin in thyroid cancer and adjacent tissues was determined by immunohistochemistry (IHC) while the Circ0005654 expression level was measured by semiquantitative real-time polymerase chain reaction (sqRT-PCR). Then, we compared the variability of Circ0005654, SP1, and Wnt/β-catenin expression in cancerous and adjacent tissues and determined the relationship between the correlation analysis and the clinicopathological features of the thyroid cancer patients. The diagnostic value of Circ0005654 in thyroid cancer tissues was analyzed with the help of the receiver operating characteristic (ROC) curve, counting the 3-year postoperative survival rate, and analyzing the effect of Circ0005654 and SP1 protein levels on the 3-year survival rate of the patients. sqRT-PCR showed that the expression level of Circ0005654 in thyroid cancer tissue was significantly higher than that of adjacent tissues. The area under the ROC of Circ0005654 was 0.9553, 95% confidence interval: (0.9211-0.9895) with a cutoff value of 0.7895, a sensitivity of 92.11%, and a specificity of 86.84%. The IHC results showed that the expression level of SP1, β-catenin, and Wnt was higher in cancer tissues than in adjacent tissues; Circ0005654, SP1, Wnt/β-catenin expression levels were associated with tumor diameter, lymph node metastasis, TNM stage, and envelope invasion (all P < .05). According to the Circ0005654 expression level in thyroid cancer tissue, the 3-year survival rate of the high expression group was 77.5% and 94.4% in the low expression group with a statistically significant difference; the 3-year survival rate of SP1 positive and negative patients was 78.6% and 100%, respectively, with the data being significantly different. Circ0005654 may serve as a potential biomarker for thyroid cancer diagnosis and may be involved in the development of thyroid cancer.
环状 RNA（CircRNAs）已被报道在各种癌症的进展中发挥关键作用，包括甲状腺癌（TC）。转录因子 1（SP1）促进甲状腺癌的发展。本研究旨在探讨Circ0005654与甲状腺癌患者中的转录因子 1（SP1）的表达水平，以用于诊断和治疗目的。共有 76 名甲状腺癌患者接受了根治性手术。术中采集甲状腺癌组织和配对的相邻组织及相应的临床病理资料。采用免疫组织化学（IHC）检测甲状腺癌和相邻组织中 SP1 和β-连环蛋白的表达，采用半定量实时聚合酶链反应（sqRT-PCR）检测 Circ0005654 的表达水平。然后，我们比较了癌症组织和相邻组织中 Circ0005654、SP1 和 Wnt/β-连环蛋白表达的变异性，并确定了甲状腺癌患者的临床病理特征之间的相关性分析。借助接收者操作特征（ROC）曲线分析 Circ0005654 在甲状腺癌组织中的诊断价值，计算 3 年术后生存率，并分析 Circ0005654 和 SP1 蛋白水平对患者 3 年生存率的影响。sqRT-PCR 显示，甲状腺癌组织中 Circ0005654 的表达水平明显高于相邻组织。Circ0005654 的 ROC 曲线下面积为 0.9553，95%置信区间：（0.9211-0.9895），截断值为 0.7895，灵敏度为 92.11%，特异性为 86.84%。免疫组化结果显示，SP1、β-连环蛋白和 Wnt 在癌组织中的表达水平高于相邻组织；Circ0005654、SP1、Wnt/β-连环蛋白表达水平与肿瘤直径、淋巴结转移、TNM 分期和包膜侵犯有关（均 P <.05）。根据甲状腺癌组织中 Circ0005654 的表达水平，高表达组的 3 年生存率为 77.5%，低表达组的 3 年生存率为 94.4%，差异有统计学意义；SP1 阳性和阴性患者的 3 年生存率分别为 78.6%和 100%，差异有统计学意义。Circ0005654 可能是甲状腺癌诊断的潜在生物标志物，可能参与甲状腺癌的发生。
