Ding Tianjiao, Zhu Yiyu, Zhou Hanjia, Zhang Zihan, Gao Erli
Department of General Surgery, The First Affiliated Hospital of Soochow University, 899 Pinghai Street, Suzhou, 215006, Jiangsu, China.
Wenzhou Medical University, Wenzhou, Zhejiang, China.
Sci Rep. 2025 Jul 1;15(1):20979. doi: 10.1038/s41598-025-05747-x.
This study aims to investigate the clinical relevance of GOLT1A in papillary thyroid carcinoma (PTC) and evaluate its significance in prognosis of PTC. In this study, 527 data downloaded from the TCGA database and 280 data downloaded from the GTEx database were used for differential gene expression analysis. Univariate and multivariate statistical analyses were performed on the data of 499 PTC patients from the TCGA database and the data of 305 PTC patients subjected to fine-needle aspiration biopsy (FNAB) from the Department of General Surgery of the First Affiliated Hospital of Soochow University and the First Affiliated Hospital of Wenzhou Medical University. In the gene expression of 527 normal tissues and thyroid carcinoma tissues data from the TCGA database and 280 normal tissues from the GTEx database, the expression of GOLT1A was significantly higher in thyroid carcinoma tissues than in normal thyroid tissues (P < 0.001), validated by GEO databases (P < 0.001) and immunohistochemical (IHC) (P < 0.05). Univariate and multivariate analyses of 499 PTC patients and 309 FNAB PTC patients in the TCGA database showed that high expression of GOLT1A was closely related to the poor prognosis of PTC. Specifically, GOLT1A is associated with differentiation risk (P = 0.005), sex (P = 0.006), capsular invasion (P = 0.038) and lateral lymph node metastasis (P < 0.001). In summary, the expression of GOLT1A in thyroid carcinoma tissues was significantly higher than that in normal tissues, and high GOLT1A expression was probably related to the poor prognosis of PTC. GOLT1A is a promising prognostic genetic marker and pathogenic target of PTC.
本研究旨在探讨GOLT1A在甲状腺乳头状癌（PTC）中的临床相关性，并评估其在PTC预后中的意义。本研究中，从TCGA数据库下载的527条数据和从GTEx数据库下载的280条数据用于差异基因表达分析。对来自TCGA数据库的499例PTC患者的数据以及来自苏州大学附属第一医院和温州医科大学附属第一医院普通外科的305例接受细针穿刺活检（FNAB）的PTC患者的数据进行单因素和多因素统计分析。在来自TCGA数据库的527例正常组织和甲状腺癌组织数据以及来自GTEx数据库的280例正常组织的基因表达中，GOLT1A在甲状腺癌组织中的表达显著高于正常甲状腺组织（P<0.001），经GEO数据库（P<0.001）和免疫组织化学（IHC）验证（P<0.05）。对TCGA数据库中的499例PTC患者和309例FNAB PTC患者进行单因素和多因素分析表明，GOLT1A的高表达与PTC的不良预后密切相关。具体而言，GOLT1A与分化风险（P=0.005）、性别（P=0.006）、包膜侵犯（P=0.038）和侧方淋巴结转移（P<0.001）相关。综上所述，GOLT1A在甲状腺癌组织中的表达显著高于正常组织，GOLT1A的高表达可能与PTC的不良预后有关。GOLT1A是一种有前景的PTC预后遗传标志物和致病靶点。
