Cullen S E, David C S, Cone J L, Sachs D H
J Immunol. 1976 Feb;116(2):549-53.
Ia antigenic specificities determined by the I-A subregion of the mouse major histocompatibility complex have been examined in strain B10.D2 (H-2d), C57BL/10 (H-2b), and in a (C57BL/6xDBA/2) hybrid (BDF1; H-2b/d). Detergent solubilized, 3H-leucine-labeled antigen preparations were mixed with appropriate alloantisera and precipitation was induced either by addition of goat anti-mouse gamma-globulin or by addition of protein A-bearing Staphylococci. Sequential precipitation analysis showed that in strain B10.D2, Ia specificities 8 and 11 were co-precipitable, and that in strain C57BL/10, Ia specificities 8 and 9 were co-precipitable. In contrast, precipitation of specificities 9 and 11 from a BDF1 antigen preparation showed that these two Ia specificities were on separate molecules. The genetic implications of these data are discussed.
已在B10.D2（H-2d）品系、C57BL/10（H-2b）品系以及（C57BL/6xDBA/2）杂交种（BDF1；H-2b/d）中检测了由小鼠主要组织相容性复合体的I-A亚区决定的Ia抗原特异性。将经去污剂溶解并用3H-亮氨酸标记的抗原制剂与适当的同种抗血清混合，通过加入山羊抗小鼠γ-球蛋白或加入携带蛋白A的葡萄球菌诱导沉淀。顺序沉淀分析表明，在B10.D2品系中，Ia特异性8和11可共同沉淀，而在C57BL/10品系中，Ia特异性8和9可共同沉淀。相比之下，从BDF1抗原制剂中沉淀特异性9和11表明，这两种Ia特异性存在于不同的分子上。讨论了这些数据的遗传学意义。
