Shippy Teresa D, Miller Sherry, Tamayo Blessy, Hosmani Prashant S, Flores-Gonzalez Mirella, Mueller Lukas A, Hunter Wayne B, Brown Susan J, D'Elia Tom, Saha Surya
Division of Biology, Kansas State University, Manhattan, KS 66506, USA.
Allen County Community College, Burlingame, KS 66413, USA.
GigaByte. 2022 Mar 17;2022:gigabyte46. doi: 10.46471/gigabyte.46. eCollection 2022.
Chitinases are enzymes that digest the polysaccharide polymer chitin. During insect development, breakdown of chitin is an essential step in molting of the exoskeleton. Knockdown of chitinases required for molting is lethal to insects, making chitinase genes an interesting target for RNAi-based pest control methods. The Asian citrus psyllid, , carries the bacterium causing Huanglongbing, or citrus greening disease, a devastating citrus disease. We identified and annotated 12 chitinase family genes from as part of a community effort to create high-quality gene models to aid the design of interdictory molecules for pest control. We categorized the chitinases according to an established classification scheme and re-evaluated the classification of chitinases in other hemipterans. In addition to chitinases from known groups, we identified a novel class of chitinases present in and several related hemipterans that appears to be the result of horizontal gene transfer.
几丁质酶是消化多糖聚合物几丁质的酶。在昆虫发育过程中，几丁质的分解是外骨骼蜕皮的关键步骤。敲除蜕皮所需的几丁质酶对昆虫是致命的，这使得几丁质酶基因成为基于RNA干扰的害虫防治方法的一个有趣靶点。亚洲柑橘木虱传播导致黄龙病（又称柑橘绿变病）的细菌，这是一种毁灭性的柑橘病害。作为创建高质量基因模型以辅助设计害虫防治阻断分子的社区努力的一部分，我们从亚洲柑橘木虱中鉴定并注释了12个几丁质酶家族基因。我们根据既定的分类方案对亚洲柑橘木虱的几丁质酶进行了分类，并重新评估了其他半翅目昆虫中几丁质酶的分类。除了已知类群的几丁质酶外，我们还在亚洲柑橘木虱和几种相关半翅目昆虫中鉴定出一类新的几丁质酶，这似乎是水平基因转移的结果。
