Brandies Parice A, Tang Simon, Johnson Robert S P, Hogg Carolyn J, Belov Katherine
School of Life and Environmental Sciences, Faculty of Science, University of Sydney, Sydney, New South Wales, Australia.
Zoologica: Veterinary and Zoological Consulting, Millthorpe, New South Wales, Australia.
GigaByte. 2020 Nov 5;2020:gigabyte7. doi: 10.46471/gigabyte.7. eCollection 2020.
Antechinus are a genus of mouse-like marsupials that exhibit a rare reproductive strategy known as semelparity and also naturally develop age-related neuropathologies similar to those in humans. We provide the first annotated antechinus reference genome for the brown antechinus (). The reference genome is 3.3 Gb in size with a scaffold N50 of 73Mb and 93.3% complete mammalian BUSCOs. Using bioinformatic methods we assign scaffolds to chromosomes and identify 0.78 Mb of Y-chromosome scaffolds. Comparative genomics revealed interesting expansions in the NMRK2 gene and the protocadherin gamma family, which have previously been associated with aging and age-related dementias respectively. Transcriptome data displayed expression of common Alzheimer's related genes in the antechinus brain and highlight the potential of utilising the antechinus as a future disease model. The valuable genomic resources provided herein will enable future research to explore the genetic basis of semelparity and age-related processes in the antechinus.
袋鼬是一种类似小鼠的有袋动物，它们展现出一种名为单次繁殖的罕见生殖策略，并且自然会发展出与人类相似的与年龄相关的神经病理学特征。我们提供了首个针对褐袋鼬（）的注释袋鼬参考基因组。该参考基因组大小为3.3Gb，支架N50为73Mb，完整的哺乳动物BUSCOs占93.3%。使用生物信息学方法，我们将支架分配到染色体上，并鉴定出0.78Mb的Y染色体支架。比较基因组学揭示了NMRK2基因和原钙黏蛋白γ家族有趣的扩增，它们之前分别与衰老和与年龄相关的痴呆症有关。转录组数据显示了袋鼬大脑中常见的阿尔茨海默病相关基因的表达，并突出了将袋鼬用作未来疾病模型的潜力。本文提供的宝贵基因组资源将使未来的研究能够探索袋鼬单次繁殖和与年龄相关过程的遗传基础。
