Doğan Hasan, Kara Aslı, Çankaya Erdem, Balkan Eda, Gürbüz Muhammet Ali, Kızılkaya Murat, Aykaç Merve
Department of Medical Biology, Faculty of Medicine, Ataturk University, Erzurum 25240, Turkey.
Department of Internal Medicine, Erzurum Regional Training and Research Hospital, Health Sciences University, Erzurum 25100, Turkey.
Curr Issues Mol Biol. 2023 Jan 19;45(2):963-974. doi: 10.3390/cimb45020062.
This prospective cross-sectional study aimed to evaluate leukocyte DNA damage in coronavirus disease (COVID-19) patients. In this study, 50 COVID-19-positive patients attending the Erzurum City Hospital Internal Medicine Outpatient Clinic and 42 control group patients were included. DNA damage was detected in living cells through leukocyte isolation in 50 COVID-19-positive patients using the comet assay method. DNA tail/head (olive) moments were evaluated and compared. White blood cells (WBC), red blood cells (RBC), hemoglobin (HGB), neutrophils (NEU), lymphocytes (LYM), eosinophils (EO), monocytes (MONO), basophils (BASO), platelets (PLT), and the neutrophil/lymphocyte ratio (NLR) were analyzed. The RBC, lymphocyte, eosinophil, and monocyte means were significantly higher in the control group ( < 0.05), whereas the HGB and neutrophile means were significantly higher in the study group ( < 0.05). There were significant negative correlations between COVID-19 and RBC (r = -0.863), LYM (r = -0.542), EO (r = -0.686), and MONO (r = -0.385). Meanwhile, there were significant positive correlations between COVID-19 and HGB (r = 0.863), NEU (r = 0.307), tail moment (r = 0.598), and olive moment (r = 0.582). Both the tail and olive moment mean differences were significantly higher in the study group, with higher ranges ( < 0.05). COVID-19 infection caused statistically significant increases in both the tail and olive damage percentage in patients, causing DNA damage. Lastly, the NLR rate was associated with the presence and progression of COVID-19.
这项前瞻性横断面研究旨在评估冠状病毒病（COVID-19）患者的白细胞DNA损伤。在本研究中，纳入了50名就诊于埃尔祖鲁姆市医院内科门诊的COVID-19阳性患者和42名对照组患者。采用彗星试验法，通过对50名COVID-19阳性患者进行白细胞分离，检测活细胞中的DNA损伤。对DNA尾/头（橄榄）矩进行评估和比较。分析了白细胞（WBC）、红细胞（RBC）、血红蛋白（HGB）、中性粒细胞（NEU）、淋巴细胞（LYM）、嗜酸性粒细胞（EO）、单核细胞（MONO）、嗜碱性粒细胞（BASO）、血小板（PLT）以及中性粒细胞/淋巴细胞比值（NLR）。对照组的红细胞、淋巴细胞、嗜酸性粒细胞和单核细胞平均值显著更高（<0.05），而研究组的血红蛋白和中性粒细胞平均值显著更高（<0.05）。COVID-19与红细胞（r = -0.863）、淋巴细胞（r = -0.542）、嗜酸性粒细胞（r = -0.686）和单核细胞（r = -0.385）之间存在显著负相关。同时，COVID-19与血红蛋白（r = 0.863）、中性粒细胞（r = 0.307）、尾矩（r = 0.598）和橄榄矩（r = 0.582）之间存在显著正相关。研究组的尾矩和橄榄矩平均差异均显著更高，范围更大（<0.05）。COVID-19感染导致患者的尾损伤百分比和橄榄损伤百分比均有统计学意义的增加，造成DNA损伤。最后，NLR率与COVID-19的存在和进展相关。
