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奥佐伊降低脂多糖诱导的结肠上皮细胞和THP-1单核细胞中的炎症反应。

Ozoile Reduces the LPS-Induced Inflammatory Response in Colonic Epithelial Cells and THP-1 Monocytes.

作者信息

Bertuccio Maria Paola, Rizzo Valentina, Arena Salvatore, Trainito Alessandra, Montalto Angela Simona, Caccamo Daniela, Currò Monica, Romeo Carmelo, Impellizzeri Pietro

机构信息

Department of Biomedical and Dental Sciences and Morpho-Functional Imaging, University of Messina, 98125 Messina, Italy.

Unit of Pediatric Surgery, Department of Human Pathology of Adult and Childhood "Gaetano Barresi", University of Messina, 98125 Messina, Italy.

出版信息

Curr Issues Mol Biol. 2023 Feb 5;45(2):1333-1348. doi: 10.3390/cimb45020087.

Abstract

Inappropriate activation of immune functions in intestinal epithelial cells can lead to inflammation that is characterized also by infiltration into intestinal tissue of monocytes/macrophages. Current therapies for intestinal inflammation include anti-inflammatory, immunosuppressive and biological drugs. Ozoile (stable ozonides) has been reported to exert anti-inflammatory effects. However, ozonated oil has been used mainly for topical applications and no data are available about its effects on intestinal cells or immune cells. In this study, we evaluated Ozoile effects on human HT-29 colonic cells and THP-1 monocytic cells stimulated with LPS to induce inflammation. HT-29 and THP-1 cells were treated with LPS in the presence/absence of Ozoile for 4 h. Biomarkers of inflammation, some members of tight junctions and the adhesion molecule ICAM were assessed by qRT-PCR. Protein expression was analyzed by Western blotting. The release of TNF-α and IL-1β was measured by ELISA. In HT-29, Ozoile inhibited LPS-induced expression of TNF-α, IL-1β, ZO-1, CLDN1, NOS2 and MMP-2 and increased the expression of Nrf2 and SOD2 antioxidant proteins. In THP-1 cells, the LPS induction of TNF-α, IL-1β and ICAM was counteracted by Ozoile treatment. Our in vitro results demonstrate the effectiveness of Ozoile in reducing the inflammatory response in intestinal and monocytic cells. Further in vivo studies are necessary to confirm its possible use for intestinal inflammatory conditions.

摘要

肠道上皮细胞免疫功能的不适当激活会导致炎症,其特征还包括单核细胞/巨噬细胞浸润到肠道组织中。目前治疗肠道炎症的方法包括抗炎药、免疫抑制剂和生物药物。据报道,奥佐利(稳定的臭氧化物)具有抗炎作用。然而,臭氧化油主要用于局部应用,关于其对肠道细胞或免疫细胞的影响尚无数据。在本研究中,我们评估了奥佐利对用脂多糖(LPS)刺激以诱导炎症的人HT-29结肠细胞和THP-1单核细胞的影响。HT-29和THP-1细胞在有/无奥佐利的情况下用LPS处理4小时。通过qRT-PCR评估炎症生物标志物、紧密连接的一些成员和黏附分子ICAM。通过蛋白质印迹分析蛋白质表达。通过ELISA测量TNF-α和IL-1β的释放。在HT-29细胞中,奥佐利抑制LPS诱导的TNF-α、IL-1β、ZO-1、CLDN1、NOS2和MMP-2的表达,并增加抗氧化蛋白Nrf2和SOD2的表达。在THP-1细胞中,奥佐利处理可抵消LPS诱导的TNF-α、IL-1β和ICAM的表达。我们的体外研究结果证明了奥佐利在减轻肠道和单核细胞炎症反应方面的有效性。需要进一步的体内研究来证实其在肠道炎症性疾病中的可能用途。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bb3/9955553/8020a52d6bfe/cimb-45-00087-g001.jpg

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