Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent B-9052, Belgium.
VIB Center for Plant Systems Biology, Ghent B-9052, Belgium.
Proc Natl Acad Sci U S A. 2023 Feb 28;120(9):e2123301120. doi: 10.1073/pnas.2123301120. Epub 2023 Feb 24.
Dehydrodiconiferyl alcohol glucoside (DCG) is a phenylpropanoid-derived plant metabolite with reported cytokinin-substituting and cell-division-promoting activity. Despite its claimed activity, DCG did not trigger morphological changes in Arabidopsis seedlings nor did it alter transcriptional shifts in cell division and cytokinin-responsive genes. In reinvestigating the bioactivity of DCG in its original setting, the previously described stimulation of tobacco callus formation could not be confirmed. No evidence was found that DCG is actually taken up by plant cells, which could explain the absence of any observable activity in the performed experiments. The DCG content in plant tissue increased when feeding explants with the DCG aglycone dehydrodiconiferyl alcohol, which is readily taken up and converted to DCG by plant cells. Despite the increased DCG content, no activity for this metabolite could be demonstrated. Our results therefore demand a reevaluation of the often-quoted cytokinin-substituting and cell-division-promoting activity that has previously been attributed to this metabolite.
去氢二松柏醇葡萄糖苷（DCG）是一种苯丙烷类植物代谢物，具有细胞分裂素替代物和促进细胞分裂的活性。尽管有报道称其具有活性，但 DCG 并未引发拟南芥幼苗形态变化，也未改变细胞分裂和细胞分裂素应答基因的转录变化。在重新研究 DCG 在其原始环境中的生物活性时，先前描述的烟草愈伤组织形成的刺激作用无法得到证实。没有证据表明 DCG 实际上被植物细胞吸收，这可以解释在进行的实验中没有观察到任何可观察到的活性。当用 DCG 的苷元去氢二松柏醇喂养外植体时，植物组织中的 DCG 含量增加，植物细胞很容易吸收并将其转化为 DCG。尽管 DCG 含量增加，但未能证明该代谢物具有活性。因此，我们的结果要求重新评估该代谢物以前常被引用的细胞分裂素替代物和促进细胞分裂的活性。
