Mansour Abdallah Tageldein, Amen Rehab M, Mahboub Heba H, Shawky Sherif M, Orabi Sahar H, Ramah Amany, Hamed Heba S
Animal and Fish Production Department, College of Agricultural and Food Sciences, King Faisal University, P.O. Box 420, Al-Ahsa 31982, Saudi Arabia; Fish and Animal Production Department, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria 21531, Egypt.
Department of Zoology, Faculty of Science, Mansoura University, Mansoura, Egypt.
Comp Biochem Physiol C Toxicol Pharmacol. 2023 May;267:109583. doi: 10.1016/j.cbpc.2023.109583. Epub 2023 Feb 22.
The present study evaluated the effect of chronic exposure to oxyfluorfen (OXY) on different physiological responses of male African catfish, Clarias gariepinus, and the ameliorative effect of Chlorella vulgaris. The fish (160 ± 5.10 g) were exposed to 1/20 LC of OXY (0.58 mg/L) for 60 consecutive days with or without co-administration of C. vulgaris (25 g/kg diet) in triplicate groups. The results revealed that chronic exposure to a sublethal level of OXY induced severe anemia and leukopenia. OXY-exposed fish experienced hypoproteinemia, marked lower AchE levels, and a significant increase in glucose, liver, and kidney function biomarkers. The DNA fragmentation of the liver increased by 15 % in fish compared to the control. On the other hand, lipid peroxidation, superoxide dismutase, and catalase activities were markedly increased in the liver and testes homogenates of the OXY-exposed fish. Meanwhile, total antioxidant capacity and glutathione S-transferase levels declined in the same tissues. Exposure to OXY induced a significant reduction in testosterone and luteinizing hormone levels and a significant increase in follicle stimulating hormone and estradiol. Meanwhile, C. vulgaris dietary supplementation succeeded in alleviating the negative impact of OXY on hematobiochemical parameters and restoring the antioxidant balance in the liver and testes. Furthermore, it ameliorated endocrine disruption and repaired sex hormone levels. In conclusion, exposure to OXY could induce systemic stress, oxidative stress, and endocrine disruption in male C. gariepinus. The dietary supplementation of C. vulgaris could be a potential protective strategy against the toxicity of OXY.
本研究评估了长期接触乙氧氟草醚（OXY）对雄性非洲鲶鱼（Clarias gariepinus）不同生理反应的影响以及普通小球藻（Chlorella vulgaris）的改善作用。将体重为（160±5.10克）的鱼分为三组，分别连续60天暴露于1/20 LC的OXY（0.58毫克/升）中，其中一组同时添加普通小球藻（25克/千克饲料），另外两组不添加。结果显示，长期接触亚致死剂量的OXY会导致严重贫血和白细胞减少。接触OXY的鱼出现低蛋白血症，乙酰胆碱酯酶水平显著降低，血糖、肝脏和肾脏功能生物标志物显著升高。与对照组相比，鱼肝脏的DNA片段化增加了15%。另一方面，接触OXY的鱼肝脏和睾丸匀浆中的脂质过氧化、超氧化物歧化酶和过氧化氢酶活性显著增加。同时，相同组织中的总抗氧化能力和谷胱甘肽S-转移酶水平下降。接触OXY导致睾酮和促黄体生成素水平显著降低，促卵泡激素和雌二醇水平显著升高。同时，添加普通小球藻饲料成功减轻了OXY对血液生化参数的负面影响，并恢复了肝脏和睾丸中的抗氧化平衡。此外，它还改善了内分泌紊乱并修复了性激素水平。总之，接触OXY会在雄性非洲鲶鱼中引发全身应激、氧化应激和内分泌紊乱。添加普通小球藻饲料可能是一种潜在的针对OXY毒性的保护策略。
