Wei Xin, Zhang Jian, Wang Bianfang, Wang Wenjia, Sun Yuqing, Li Ling, Xu Hai, Wang Mingyu
State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, Qingdao 266237, China.
Biology (Basel). 2023 Jan 29;12(2):210. doi: 10.3390/biology12020210.
This work aims to investigate the impact of antibiotics and extracellular antibiotic resistance genes (eARGs) on the dynamics of gastrointestinal antimicrobial resistance (AMR). The antibiotic resistance gene (ARG) levels of different segments of the gastrointestinal tract of mouse models were analyzed and compared after exposure to clinical concentrations of sulfadiazine and environmental levels of eARGs carried by the conjugative plasmid pR55. Exposure to sulfadiazine and eARGs led to significant changes in ARG levels by as many as four log-folds. Further analysis showed that the response of ARG levels appeared from 12-16 days after exposure and diminished 20 days after exposure. The responses in ARG levels were also restricted to different gastrointestinal segments for sulfadiazine and eARGs. Combined exposure of sulfadiazine and eARGs was unable to further increase ARG levels. From these findings, we concluded that the short-term consumption of environmental levels of eARGs and uptake of clinical levels of antibiotics lead to a spatially and temporally confined response in gastrointestinal AMR. These findings further clarify the detrimental impacts of antibiotic and eARG uptake, and the complexity of AMR development and dissemination dynamics in the gastrointestinal tract.
这项工作旨在研究抗生素和细胞外抗生素抗性基因（eARGs）对胃肠道抗菌药物耐药性（AMR）动态变化的影响。在小鼠模型的胃肠道不同节段暴露于临床浓度的磺胺嘧啶以及接合质粒pR55携带的环境水平eARGs之后，分析并比较了抗生素抗性基因（ARG）水平。暴露于磺胺嘧啶和eARGs导致ARG水平发生多达四个对数级的显著变化。进一步分析表明，ARG水平的响应在暴露后12 - 16天出现，并在暴露后20天减弱。对于磺胺嘧啶和eARGs，ARG水平的响应也局限于不同的胃肠道节段。磺胺嘧啶和eARGs的联合暴露无法进一步提高ARG水平。基于这些发现，我们得出结论，短期摄入环境水平的eARGs以及摄取临床水平的抗生素会导致胃肠道AMR在空间和时间上受限的响应。这些发现进一步阐明了抗生素和eARG摄取的有害影响，以及胃肠道中AMR发展和传播动态的复杂性。
