Baudequin Timothée, Wee Hazel, Cui Zhanfeng, Ye Hua
Department of Engineering Science, Institute of Biomedical Engineering, University of Oxford, Oxford OX3 7DQ, UK.
Biomechanics and Bioengineering, CNRS, Centre de Recherche Royallieu, Université de Technologie de Compiègne, CS 60 319, 60203 Compiègne, France.
Bioengineering (Basel). 2023 Jan 26;10(2):163. doi: 10.3390/bioengineering10020163.
Micro-carriers, thanks to high surface/volume ratio, are widely studied as mesenchymal stem cell (MSCs) in vitro substrate for proliferation at clinical rate. In particular, Ca-alginate-based biomaterials (sodium alginate crosslinked with CaCl) are commonly investigated. However, Ca-alginate shows low bioactivity and requires functionalization, increasing labor work and costs. In contrast, films of sodium alginate crosslinked with iron chloride (Fe-alginate) have shown good bioactivity with fibroblasts, but MSCs studies are lacking. We propose a first proof-of-concept study of Fe-alginate beads supporting MSCs proliferation without functionalization. Macro- and micro-carriers were prepared (extrusion and electrospray) and we report for the first time Fe-alginate electrospraying optimization. FTIR spectra, stability with various mannuronic acids/guluronic acids (M/G) ratios and size distribution were analyzed before performing cell culture. After confirming literature results on films with human MSCs, we showed that Macro-Fe-alginate beads offered a better environment for MSCs adhesion than Ca-alginate. We concluded that Fe-alginate beads showed great potential as ready-to-use carriers.
微载体由于具有高的表面积/体积比，作为间充质干细胞（MSCs）在临床速率下进行增殖的体外基质受到广泛研究。特别是，基于钙藻酸盐的生物材料（与氯化钙交联的海藻酸钠）被普遍研究。然而，钙藻酸盐显示出低生物活性且需要功能化，这增加了劳动量和成本。相比之下，与氯化铁交联的海藻酸钠薄膜（铁藻酸盐）已显示出对成纤维细胞具有良好的生物活性，但缺乏对MSCs的研究。我们提出了一项关于铁藻酸盐珠支持MSCs增殖而无需功能化的初步概念验证研究。制备了宏观和微观载体（挤压和电喷雾），并且我们首次报道了铁藻酸盐电喷雾的优化。在进行细胞培养之前，分析了傅里叶变换红外光谱、不同甘露糖醛酸/古洛糖醛酸（M/G）比例下的稳定性以及尺寸分布。在确认了关于人MSCs薄膜的文献结果后，我们表明宏观铁藻酸盐珠为MSCs粘附提供了比钙藻酸盐更好的环境。我们得出结论，铁藻酸盐珠作为即用型载体具有巨大潜力。
