Schmidt Anke, Mühl Melissa, Brito Walison Augusto da Silva, Singer Debora, Bekeschus Sander
ZIK Plasmatis, Leibniz Institute for Plasma Science and Technology (INP), Felix-Hausdorff-Str. 2, 17489 Greifswald, Germany.
Department of General Pathology, State University of Londrina, Rodovia Celso Garcia Cid, Londrina 86020-000, Brazil.
Antioxidants (Basel). 2023 Jan 18;12(2):227. doi: 10.3390/antiox12020227.
Polystyrene nano- and micro-sized plastic particles (NMP) are one of the common plastic materials produced that dramatically pollute the environment, water, and oceanic habitats worldwide. NMP are continuously absorbed by the body through a number of routes, especially via intestinal ingestion, dermal uptake, and inhalation into the lung. Several studies provided evidence of NMP provoking oxidative stress and affecting cellular responses. Yet, the NMP effects on primary lung cells have not been studied. To this end, we isolated and cultured murine lung cells and exposed them short-term or long-term to polystyrene 0.2-6.0 µm-sized NMP. We studied cellular consequences regarding oxidative stress, morphology, and secretion profiling. Visualization, distribution, and expression analyses confirmed lung cells accumulating NMP and showed several significant correlations with particle size. Moreover, we found substantial evidence of biological consequences of small-scale NMP uptake in lung cells. Besides alterations of cytokine secretion profiles resulting in inflammatory responses, indicators of oxidative stress were identified that were accompanied by Nrf2 and β-catenin signaling changes. Our results serve as an important basis to point out the potential hazards of plastic contaminations and uptake in lung cells.
聚苯乙烯纳米和微米级塑料颗粒（NMP）是生产的常见塑料材料之一，它们在全球范围内对环境、水和海洋栖息地造成了严重污染。NMP通过多种途径不断被人体吸收，尤其是通过肠道摄入、皮肤吸收和吸入肺部。多项研究提供了NMP引发氧化应激并影响细胞反应的证据。然而，NMP对原代肺细胞的影响尚未得到研究。为此，我们分离并培养了小鼠肺细胞，并将它们短期或长期暴露于0.2 - 6.0微米大小的聚苯乙烯NMP中。我们研究了氧化应激、形态学和分泌谱方面的细胞后果。可视化、分布和表达分析证实肺细胞积累了NMP，并显示出与颗粒大小的若干显著相关性。此外，我们发现了肺细胞摄取小规模NMP产生生物学后果的大量证据。除了导致炎症反应的细胞因子分泌谱改变外，还确定了氧化应激指标，同时伴有Nrf2和β-连环蛋白信号变化。我们的结果为指出塑料污染和肺细胞摄取的潜在危害提供了重要依据。
