Zhong Min, Yue Lingqi, Liu Wei, Qin Hongyi, Lei Bingfu, Huang Riming, Yang Xian, Kang Yunyan
College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
Key Laboratory for Biobased Materials and Energy of Ministry of Education, Guangdong Provincial Engineering Technology Research Center for Optical Agriculture, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China.
Antioxidants (Basel). 2023 Jan 18;12(2):228. doi: 10.3390/antiox12020228.
The polyamine uptake transporter (Put), an important polyamines-related protein, is involved in plant cell growth, developmental processes, and abiotic stimuli, but no research on the Put family has been carried out in the tomato. Herein, eight tomato Put were identified and scattered across four chromosomes, which were classified into three primary groups by phylogenetic analysis. Protein domains and gene structural organization also showed a significant degree of similarity, and the genes were significantly induced by various hormones and polyamines. Tissue-specific expression analysis indicated that genes were expressed in all tissues of the tomato. The majority of genes were induced by different abiotic stresses. Furthermore, transcription was found to be responsive to salt stress, and overexpression of in yeast conferred salinity tolerance and polyamine uptake. Moreover, overexpression of in tomatoes promoted salinity tolerance accompanied by a decrease in the Na/K ratio, restricting the generation of reactive oxygen and increasing polyamine metabolism and catabolism, antioxidant enzyme activity (SOD, CAT, APX, and POD), and nonenzymatic antioxidant activity (GSH/GSSG and ASA/DHA ratios, GABA, and flavonoid content); loss of function of produced opposite effects. These findings highlight that Put2 plays a pivotal role in mediating polyamine synthesis and catabolism, and the antioxidant capacity in tomatoes, providing a valuable gene for salinity tolerance in plants.
多胺摄取转运蛋白（Put）是一种重要的与多胺相关的蛋白质，参与植物细胞生长、发育过程和非生物刺激，但尚未在番茄中对Put家族进行研究。在此，鉴定出8个番茄Put基因，它们分布在4条染色体上，通过系统发育分析分为3个主要组。蛋白质结构域和基因结构组织也显示出显著的相似性，并且这些基因受到各种激素和多胺的显著诱导。组织特异性表达分析表明，这些基因在番茄的所有组织中均有表达。大多数基因受到不同非生物胁迫的诱导。此外，发现Put2的转录对盐胁迫有反应，但在酵母中过表达Put2可赋予耐盐性和多胺摄取能力。此外，在番茄中过表达Put2可提高耐盐性，同时降低Na/K比值，限制活性氧的产生，增加多胺代谢和分解代谢、抗氧化酶活性（超氧化物歧化酶、过氧化氢酶、抗坏血酸过氧化物酶和过氧化物酶）以及非酶抗氧化活性（谷胱甘肽/氧化型谷胱甘肽和抗坏血酸/脱氢抗坏血酸比值、γ-氨基丁酸和类黄酮含量）；Put2功能丧失则产生相反的效果。这些发现突出表明Put2在介导番茄多胺合成和分解代谢以及抗氧化能力方面起着关键作用，为植物耐盐性提供了一个有价值的基因。
