利用CRISPR/Cas9基因编辑技术破坏水稻中的三个多胺摄取转运蛋白基因可赋予对除草剂百草枯的耐受性。
Disruption of three polyamine uptake transporter genes in rice by CRISPR/Cas9 gene editing confers tolerance to herbicide paraquat.
作者信息
Lyu Yu-Shu, Cao Li-Miao, Huang Wen-Qian, Liu Jian-Xiang, Lu Hai-Ping
机构信息
State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058 China.
State Key Laboratory of Rice Biology, Zhejiang Provincial Key Laboratory of Crop Genetic Resources, Institute of Crop Science, Zhejiang University, Hangzhou, 310058 China.
出版信息
aBIOTECH. 2022 Jun 25;3(2):140-145. doi: 10.1007/s42994-022-00075-4. eCollection 2022 Jun.
UNLABELLED
Weeds are a major biotic constraint that can cause dramatic crop production losses. Herbicide technology has been widely used by farmers as the most cost-effective weed control measure, and development of new strategy to improve herbicide tolerance in plants is urgently needed. The CRISPR/Cas9-based genome editing tool has been used in diverse applications related to agricultural technology for crop improvement. Here we identified three polyamine uptake transporter () genes in rice that are homologous to the At. We successfully demonstrate that CRISPR/Cas9-targeted mutagenesis of greatly improves paraquat resistance in rice without obvious yield penalty. Therefore, manipulation of these loci could be valuable for producing transgene-free rice with improved herbicide resistance in future.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s42994-022-00075-4.
未标注
杂草是一种主要的生物限制因素，可导致农作物产量大幅损失。除草剂技术已被农民广泛用作最具成本效益的杂草控制措施，因此迫切需要开发提高植物除草剂耐受性的新策略。基于CRISPR/Cas9的基因组编辑工具已用于与农业技术相关的各种作物改良应用。在这里，我们在水稻中鉴定出三个与拟南芥同源的多胺转运体（）基因。我们成功证明，对进行CRISPR/Cas9靶向诱变可大大提高水稻对百草枯的抗性，且不会对产量造成明显损失。因此，操纵这些基因座对于未来生产具有改良除草剂抗性的无转基因水稻可能具有重要价值。
补充信息
在线版本包含可在10.1007/s42994-022-00075-4获取的补充材料。
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