Li Chen, Cao Yiran, Kohei Fukuda, Hao Haihong, Peng Guiqing, Cheng Can, Ye Jing
State Key Laboratory of Agricultural Microbiology, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China.
Wuhan Britain-China School, Wuhan, 430070, China.
Virol Sin. 2022 Apr;37(2):277-283. doi: 10.1016/j.virs.2022.01.023. Epub 2022 Jan 22.
Since the anti-inflammatory effect of hydrogen has been widely known, it was supposed that hydrogen could suppress tissue damage by inhibiting virus-related inflammatory reactions. However, hydrogen is slightly soluble in water, which leads to poor effect of oral hydrogen-rich water therapy. In this study, the nano-bubble hydrogen water (nano-HW) (about 0.7 ppm) was prepared and its therapeutic effect against viral infection was investigated by utilizing spring viraemia of carp virus (SVCV)-infected zebrafish as model. Three-month-old zebrafish were divided into nano-HW treatment-treated group and aquaculture water treated group (control group). The results revealed that the cumulative mortality rate of SVCV-infected zebrafish was reduced by 40% after treatment with nano-bubble hydrogen water, and qRT-PCR results showed that SVCV replication was significantly inhibited. Histopathological examination staining showed that SVCV infection caused tissue damage was greatly alleviated after treatment with nano-bubble hydrogen water. Futhermore, SVCV infection caused reactive oxygen species (ROS) accumulation was significantly reduced upon nano-HW treatment. The level of proinflammatory cytokines IL-1β, IL-8, and TNF-α was remarkably reduced in the nano-HW-treated group in vivo and in vitro. Taken together, our data demonstrated for the first time that nano-HW could inhibit the inflammatory response caused by viral infection in zebrafish, which suggests that nano-HW can be applied to antiviral research，and provides a novel therapeutic strategy for virus-caused inflammation related disease.
由于氢气的抗炎作用已广为人知，人们推测氢气可通过抑制病毒相关的炎症反应来抑制组织损伤。然而，氢气在水中的溶解度较低，这导致口服富氢水疗法的效果不佳。在本研究中，制备了纳米气泡氢水（nano-HW）（约0.7 ppm），并以感染鲤春病毒血症病毒（SVCV）的斑马鱼为模型，研究了其对病毒感染的治疗效果。将3个月大的斑马鱼分为纳米气泡氢水处理组和养殖水处理组（对照组）。结果显示，用纳米气泡氢水处理后，感染SVCV的斑马鱼的累积死亡率降低了40%，qRT-PCR结果表明SVCV复制受到显著抑制。组织病理学检查染色显示，用纳米气泡氢水处理后，SVCV感染引起的组织损伤得到了极大缓解。此外，纳米气泡氢水处理后，SVCV感染引起的活性氧（ROS）积累显著减少。在体内和体外，纳米气泡氢水处理组中促炎细胞因子IL-1β、IL-8和TNF-α的水平均显著降低。综上所述，我们的数据首次证明纳米气泡氢水可以抑制斑马鱼病毒感染引起的炎症反应，这表明纳米气泡氢水可应用于抗病毒研究，并为病毒引起的炎症相关疾病提供了一种新的治疗策略。
