Polo-Castellano Curro, Mateos Rosa María, Visiedo Francisco, Palma Miguel, Barbero Gerardo F, Ferreiro-González Marta
Department of Analytical Chemistry, Faculty of Sciences, Agrifood Campus of International Excellence (ceiA3), Wine and Food Research Institute (IVAGRO), University of Cadiz, 11510 Puerto Real, Spain.
Research Unit, Biomedical Research and Innovation Institute of Cadiz (INiBICA), Puerta del Mar University Hospital, 11009 Cadiz, Spain.
Antioxidants (Basel). 2023 Feb 3;12(2):369. doi: 10.3390/antiox12020369.
Lam. is known to have significant antioxidant properties. Because of this, the development of an optimal extraction method is crucial to obtain pharmacological products based on the bioactive compounds produced by this tree. Through a Plackett-Burman and a Box-Behnken design, enzymatic extraction conditions (temperature, agitation, solvent pH and composition, sample-to-solvent ratio, enzyme-to-sample ratio and extraction time) have been optimized using normalized areas (UA/g) as response variable and relative mass (mg/g) as quantification variable. Extractions were performed in an incubator, where all the extraction conditions could be digitally controlled. Thus, 58.9 °C, 50 rpm, 4.0 pH, 32.5% EtOH, 0.2 g sample in 15 mL solvent and 106 U/g were established as the optimal extraction conditions for the extraction with a mix of pectinases coming from . Under these optimal conditions, two-minute extractions were performed and evaluated through a single factor design. The enzymatic extraction method demonstrated its suitability to produce extracts with good antioxidant power (antioxidant activity 4.664 ± 0.059 mg trolox equivalent/g sample and total phenolic compounds 6.245 ± 0.101 mg gallic acid equivalent/g sample). The method was also confirmed to have good repeatability (1.39%) and intermediate precision (2.37%) levels.
已知榄李具有显著的抗氧化特性。因此，开发一种优化的提取方法对于基于该树种产生的生物活性化合物获得药理产品至关重要。通过Plackett-Burman设计和Box-Behnken设计，以归一化面积（UA/g）作为响应变量、相对质量（mg/g）作为定量变量，对酶提取条件（温度、搅拌、溶剂pH值和组成成分以及样品与溶剂的比例、酶与样品的比例和提取时间）进行了优化。提取在培养箱中进行，所有提取条件均可数字控制。因此，确定58.9℃、50转/分钟、pH值4.0、32.5%乙醇、15毫升溶剂中0.2克样品以及106 U/g为使用来自……的果胶酶混合物进行提取的最佳提取条件。在这些最佳条件下，进行两分钟提取，并通过单因素设计进行评估。酶提取方法证明其适合生产具有良好抗氧化能力的提取物（抗氧化活性为4.664±0.059毫克特洛克斯当量/克样品，总酚类化合物为6.245±0.101毫克没食子酸当量/克样品）。该方法还被证实具有良好的重复性（1.39%）和中间精密度（2.37%）水平。
