Kheyar Farida, Kheyar Naoual, Amiali Malek, Boulekbache-Makhlouf Lila, Kadi Ahcene, Benchabane Ahmed, Bitam Arezki
Research Laboratory in Food Technology and Human Nutrition (LRTANH), National Higher School of Agronomics El- Harrach (ENSA, ES1603), Algiers, Algeria.
Laboratory of Plant Biotechnology and Ethnobotany, University of Bejaia, Bejaia, Algeria.
Nat Prod Res. 2024 Mar 23:1-9. doi: 10.1080/14786419.2024.2333044.
The study aimed to optimise the microwave-assisted extraction (MAE) of total phenolic compounds (TPC) from Lam. leaves using response surface methodology (RSM) and Box-Behnken designs (BBD). Factors such as ethanol concentration, microwave power, irradiation time, and solvent to solid ratio were assessed in single-factor tests. Under optimal extraction conditions (48.86% ethanol, 626.53 W microwave power, 99.48 s irradiation, 29.67 mL/g solvent to solid ratio and 21.12 W/mL power density), TPC was 58.45 ± 0.68 mg GAE/g DW, close to the predicted 59.78 ± 1.47 mg GAE/g DW. The MAE method outperformed maceration extraction (8.41 ± 0.25 mg GAE/g DW) and decoction extraction (38.74 ± 0.81 mg GAE/g DW) in terms of extraction time, solvent use, and efficiency. The optimised extract exhibited significant antioxidant activity with IC50 values of 0.294 ± 0.004 mg/mL (DPPH) and 0.425 ± 0.005 mg/mL (ABTS), confirming the efficacy of MAE in preparing antioxidant-rich plant extracts.
本研究旨在利用响应面法（RSM）和Box-Behnken设计（BBD）优化从Lam.叶中微波辅助提取（MAE）总酚化合物（TPC）的工艺。在单因素试验中评估了乙醇浓度、微波功率、辐照时间和溶剂与固体比等因素。在最佳提取条件下（乙醇浓度48.86%、微波功率626.53W、辐照99.48s、溶剂与固体比29.67mL/g、功率密度21.12W/mL），TPC为58.45±0.68mg GAE/g DW，接近预测的59.78±1.47mg GAE/g DW。在提取时间、溶剂使用和效率方面，MAE方法优于浸渍提取（8.41±0.25mg GAE/g DW）和煎煮提取（38.74±0.81mg GAE/g DW）。优化后的提取物表现出显著的抗氧化活性，DPPH的IC50值为0.294±0.004mg/mL，ABTS的IC50值为0.425±0.005mg/mL，证实了MAE在制备富含抗氧化剂的植物提取物方面的有效性。
