Hirano Y, Shibahara H, Obara H, Suzuki T, Takamizawa S, Yamaguchi C, Tsunoda H, Sato I
Department of Obstetrics and Gynecology, Jichi Medical School, 3311-1, Yakushiji, Minamikawachi-machi, Tochigi 329-0498, Japan.
J Assist Reprod Genet. 2001 Apr;18(4):213-8. doi: 10.1023/a:1009420432234.
Some studies have suggested that computer-aided sperm analysis (CASA) estimates of concentration and movement characteristics of progressively motile spermatozoa are related to fertilization rates in vitro. However, it has also been suggested that the greater number of motility parameters assessed by CASA does not imply more precision in predicting fertility. This study was carried out to investigate the relationships between the CASA estimates and fertilization rates in vitro.
Semen quality analysis was performed using CASA in 136 in vitro fertilization-embryo transfer (IVF-ET) cycles with at least 3 oocytes collected. The CASA estimates before and after swim-up were compared between 108 cycles with fertilization rate > 50% ("good" group) and 28 cycles with fertilization rate < or = 50% ("poor" group).
Before swim-up, there were significant correlations between fertilization rates and CASA estimates, including amplitude of lateral head displacement (ALH) (r = .269), curvilinear velocity (VCL) (r = .297), straight line velocity (VSL) (r = .266), and rapid sprm movement (Rapid) (r = .243). There was also a significant correlation between the fertilization rates and straightness (STR) after swim-up (r = -0.178). As for sperm movement characteristics, there were significant differences of ALH (p < .005), VCL (p < .001), VSL (p < .005), and Rapid (p < .01) between "good" and "poor" groups before swim-up. After swim-up, there were significant differences of VCL (p < .005), average path velocity (VAP) (p < .005), and Rapid (p < .05) between the two groups.
These results indicate that some of the CASA estimates provide reliable estimation of the fertilizing ability of human sperm. There were significant differences of the two sperm movement characteristics, including VCL and Rapid (before and after swim-up), indicating that the total distance traveled by rapid sperm movement might be important in human sperm fertilizing abilities.
一些研究表明，计算机辅助精子分析（CASA）对渐进性活动精子浓度和运动特征的估计与体外受精率相关。然而，也有人提出，CASA评估的更多运动参数并不意味着在预测生育能力方面更精确。本研究旨在探讨CASA估计值与体外受精率之间的关系。
在136个至少采集到3个卵母细胞的体外受精-胚胎移植（IVF-ET）周期中，使用CASA进行精液质量分析。比较了受精率>50%的108个周期（“良好”组）和受精率≤50%的28个周期（“较差”组）上游前后的CASA估计值。
上游前，受精率与CASA估计值之间存在显著相关性，包括头部侧向位移幅度（ALH）（r = 0.269）、曲线速度（VCL）（r = 0.297）、直线速度（VSL）（r = 0.266）和快速精子运动（Rapid）（r = 0.243）。上游后，受精率与直线性（STR）之间也存在显著相关性（r = -0.178）。关于精子运动特征，“良好”组和“较差”组上游前的ALH（p < 0.005）、VCL（p < 0.001）、VSL（p < 0.005）和Rapid（p < 0.01）存在显著差异。上游后，两组之间的VCL（p < 0.005）、平均路径速度（VAP）（p < 0.005）和Rapid（p < 0.05）存在显著差异。
这些结果表明，一些CASA估计值能可靠地评估人类精子的受精能力。两种精子运动特征存在显著差异，包括上游前后的VCL和Rapid，这表明快速精子运动的总行程可能对人类精子受精能力很重要。
