Pan Ziyi, Li Xuewen, Wu Dongsheng, Chen Xingyong, Zhang Cheng, Jin Sihua, Geng Zhaoyu
College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
Animals (Basel). 2023 Feb 15;13(4):680. doi: 10.3390/ani13040680.
The accumulation of fat in ducks is the main cause of low feed efficiency and metabolic diseases in ducks. Retinoic acid X receptor alpha () is a member of the nuclear receptor superfamily involved in lipid, glucose, energy, and hormone metabolism. The effect of the gene on lipid metabolism in duck preadipocytes (DPACs) and the relationship between SNPs and the feed efficiency traits of ducks are unclear.
qRT-PCR and Western blotting analyses were used to detect changes in mRNA and protein in cells. Intracellular triglycerides (TGs) were detected using an ELISA kit. A general linear model analysis was used to determine the association between SNPs and feed efficiency.
The duck gene was highly expressed on the fourth day of DPAC differentiation. The gene increased the content of fat and TG in DPACs and promoted the expression of cell differentiation genes; g.5,952,667 correlated with average daily feed intake (ADFI), residual feed intake (RFI), and feed conversion ratio (FCR).
Duck can accelerate fat accumulation, and the polymorphism of the gene is closely related to feed efficiency, which provides basic data for breeding high feed efficiency ducks.
鸭体内脂肪的积累是鸭饲料效率低下和代谢疾病的主要原因。视黄酸X受体α（ ）是核受体超家族的成员，参与脂质、葡萄糖、能量和激素代谢。 基因对鸭前脂肪细胞（DPACs）脂质代谢的影响以及单核苷酸多态性（SNPs）与鸭饲料效率性状之间的关系尚不清楚。
采用qRT-PCR和蛋白质免疫印迹分析检测细胞中mRNA和蛋白质的变化。使用酶联免疫吸附测定（ELISA）试剂盒检测细胞内甘油三酯（TGs）。采用一般线性模型分析确定 SNPs与饲料效率之间的关联。
鸭 基因在DPAC分化的第4天高度表达。 基因增加了DPACs中脂肪和TG的含量，并促进了细胞分化基因的表达；g.5,952,667与平均日采食量（ADFI）、剩余采食量（RFI）和饲料转化率（FCR）相关。
鸭 可加速脂肪积累，且 基因的多态性与饲料效率密切相关，这为培育高饲料效率的鸭提供了基础数据。
