Yang Xin, Xian Yuanrong, Li Zhenhui, Wang Zhijun, Nie Qinghua
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Lingnan Guangdong Laboratory of Agriculture, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
National-Local Joint Engineering Research Center for Livestock Breeding, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, China.
Animals (Basel). 2022 Apr 2;12(7):916. doi: 10.3390/ani12070916.
Gene single nucleotide polymorphisms can be used as auxiliary markers in molecular breeding and are an effective method to improve production performance. G0S2 is a key gene involved in regulating fat metabolism, but little research has been conducted on this gene regarding its role in poultry. In this study, the specialized commercial partridge chicken strain G0S2 gene was cloned and sequenced, and the relationship between the SNP sites on G0S2 and the carcass traits of chickens was investigated. The results showed that a total of seven SNPs were detected on G0S2 (g.102G > A, g.255G > A, g.349C > T, g.384A > G, g.386G > A, g.444G > A, g.556G > A). Two sites are located in the coding region and five sites are located in the 3′-UTR. SNPs located in the coding region are synonymous mutations. g.444G > A has a significant correlation with abdominal fat weight. The chickens with AG and GG genotypes have the highest abdominal fat weight, while the AA genotype is lower. The g.102G > A genotype has a significant correlation with live and abdominal fat weight. The live weight and abdominal fat weight of the chickens with AA and AG genotypes are at a higher level and have a larger gap than the GG genotype. Chickens with the AA genotype in g.556G > A had the lowest fat weight. The results of present study can provide practical information for molecular marker-assisted breeding of chicken carcass traits.
基因单核苷酸多态性可作为分子育种中的辅助标记，是提高生产性能的有效方法。G0S2是参与调节脂肪代谢的关键基因，但关于该基因在家禽中的作用研究较少。本研究对专用商业鹧鸪鸡品系的G0S2基因进行了克隆和测序，并研究了G0S2上的SNP位点与鸡胴体性状之间的关系。结果表明，在G0S2上共检测到7个SNP（g.102G>A、g.255G>A、g.349C>T、g.384A>G、g.386G>A、g.444G>A、g.556G>A）。两个位点位于编码区，五个位点位于3′-UTR。位于编码区的SNP为同义突变。g.444G>A与腹脂重显著相关。AG和GG基因型的鸡腹脂重最高，而AA基因型较低。g.102G>A基因型与活重和腹脂重显著相关。AA和AG基因型的鸡的活重和腹脂重处于较高水平，且与GG基因型相比差距更大。g.556G>A中AA基因型的鸡脂肪重量最低。本研究结果可为鸡胴体性状的分子标记辅助育种提供实用信息。
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