Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
Department of Microbiology, University of Helsinki, Viikinkaari 9, 00790 Helsinki, Finland.
Biomolecules. 2023 Jan 17;13(2):188. doi: 10.3390/biom13020188.
l-arabitol is an intermediate of the pentose catabolic pathway in fungi but can also be used as a carbon source by many fungi, suggesting the presence of transporters for this polyol. In this study, an l-arabitol transporter, LatA, was identified in . Growth and expression profiles as well as sugar consumption analysis indicated that LatA only imports l-arabitol and is regulated by the arabinanolytic transcriptional activator AraR. Moreover, l-arabitol production from wheat bran was increased in a metabolically engineered mutant by the deletion of , indicating its potential for improving l-arabitol-producing cell factories. Phylogenetic analysis showed that homologs of LatA are widely conserved in fungi.
l-阿拉伯糖醇是真菌戊糖分解代谢途径的中间产物，但也可以被许多真菌用作碳源，这表明存在这种多元醇的转运蛋白。在这项研究中，鉴定出一种 l-阿拉伯糖醇转运蛋白 LatA 存在于 。生长和表达谱以及糖消耗分析表明，LatA 仅导入 l-阿拉伯糖醇，并受阿拉伯聚糖水解转录激活因子 AraR 调控。此外，通过缺失 ，在代谢工程 突变体中增加了从小麦麸皮中生产 l-阿拉伯糖醇，表明其有可能提高 l-阿拉伯糖醇生产细胞工厂的生产能力。系统发育分析表明，LatA 的同源物在真菌中广泛保守。
