Kim Ji Hyeon, Seo Jeonghyeon, No Huiwon, Kuge Takao, Mori Takahiro, Kimoto Hisashi, Kim Jin-Kyung
Department of Biomedical Science, Daegu Catholic University, Gyeongsan-si 38430, Republic of Korea.
ADEKA Corporation, R&D Division, 7-2-34, Higashi-Ogu, Arakawa-ku, Tokyo 116-8553, Japan.
Biomedicines. 2023 Feb 11;11(2):529. doi: 10.3390/biomedicines11020529.
β-glucan, a plant polysaccharide, mainly exists in plant cell walls of oats, barley, and wheat. It is attracting attention due to its high potential for use as functional foods and pharmaceuticals. We have previously reported that low-molecular-weight -fermented β-D-glucan (LMW-AP-FBG) could inhibit inflammatory responses by inhibiting mitogen-activated protein kinases and nuclear factor-κB signaling pathways. Bases on previous results, the objective of the present study was to investigate the therapeutic potential of LMW-AP-FBG in BALB/c mice intracutaneously transplanted with CT-26 colon cancer cells onto their backs. Daily intraperitoneal injections of LMW-AP-FBG (5 mg/kg) for two weeks significantly suppressed tumor growth in mice bearing CT-26 tumors by reducing tumor proliferation and inducing apoptosis as compared to phosphate buffer-treated control mice. In addition, LMW-AP-FBG treatment reduced the viability of CT-26 cells in a dose-dependent manner by inducing apoptosis with loss of mitochondrial transmembrane potential and increased activated caspases. Taken together, LMW-AP-FBG exhibits anticancer properties both in vivo and in vitro.
β-葡聚糖是一种植物多糖，主要存在于燕麦、大麦和小麦的植物细胞壁中。由于其作为功能性食品和药物的巨大潜力而备受关注。我们之前报道过，低分子量发酵β-D-葡聚糖（LMW-AP-FBG）可通过抑制丝裂原活化蛋白激酶和核因子-κB信号通路来抑制炎症反应。基于之前的研究结果，本研究的目的是探讨LMW-AP-FBG对背部皮下移植CT-26结肠癌细胞的BALB/c小鼠的治疗潜力。与磷酸盐缓冲液处理的对照小鼠相比，连续两周每天腹腔注射LMW-AP-FBG（5毫克/千克）可通过减少肿瘤增殖和诱导凋亡，显著抑制携带CT-26肿瘤的小鼠的肿瘤生长。此外，LMW-AP-FBG处理通过诱导线粒体跨膜电位丧失和增加活化的半胱天冬酶来诱导凋亡，从而以剂量依赖的方式降低CT-26细胞的活力。综上所述，LMW-AP-FBG在体内和体外均表现出抗癌特性。
