School of Chemical Engineering, Clean Energy Research Center, Jeonbuk National University, Jeonju 54896, Republic of Korea.
Biosensors (Basel). 2023 Jan 21;13(2):171. doi: 10.3390/bios13020171.
Proteolytic enzymes are one of the important biomarkers that enable the early diagnosis of several diseases, such as cancers. A specific proteolytic enzyme selectively degrades a certain sequence of a polypeptide. Therefore, a particular proteolytic enzyme can be selectively quantified by changing detectable signals causing degradation of the peptide chain. In addition, by combining polypeptides with various functional nanomaterials, proteolytic enzymes can be measured more sensitively and rapidly. In this paper, proteolytic enzymes that can be measured using a polypeptide degradation method are reviewed and recently studied functional nanomaterials-based proteolytic biosensors are discussed. We anticipate that the proteolytic nanobiosensors addressed in this review will provide valuable information on physiological changes from a cellular level for individual and early diagnosis.
蛋白水解酶是一类重要的生物标志物，可以用于多种疾病（如癌症）的早期诊断。一种特定的蛋白水解酶可以选择性地降解多肽的特定序列。因此，通过改变导致肽链降解的可检测信号，可以选择性地定量特定的蛋白水解酶。此外，通过将多肽与各种功能纳米材料结合，可以更灵敏、快速地测量蛋白水解酶。本文综述了可通过多肽降解方法进行测量的蛋白水解酶，并讨论了最近研究的基于功能纳米材料的蛋白水解生物传感器。我们预计，本文中讨论的蛋白水解纳米生物传感器将为个体和早期诊断提供来自细胞水平的生理变化的有价值信息。
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