Chemistry Faculty of M.V. Lomonosov, Moscow State University, 119991 Moscow, Russia.
Biosensors (Basel). 2023 Feb 9;13(2):250. doi: 10.3390/bios13020250.
We report on hydrogen peroxide sensors made through a single printing step with carbon ink containing catalytically synthesized Prussian blue nanoparticles. Despite their reduced sensitivity, the resulting bulk-modified sensors displayed both a wider linear calibration range (5 × 10-1 × 10 M) and an approximately four times lower detection limit versus the surface-modified sensors due to the dramatically decreased noise resulting in, on average, a six times higher signal-to-noise ratio. The corresponding glucose and lactate biosensors demonstrated similar and even higher sensitivities compared to those of biosensors based on surface-modified transducers. The biosensors have been validated through analysis of human serum. The decreased time and cost for production of single printing step bulk-modified transducers, as well as their analytical performance characteristics, which are advantageous over conventional surface-modified ones, would be expected to enable their wide use in (bio)sensorics.
我们报告了一种通过使用含有催化合成普鲁士蓝纳米粒子的碳墨水进行单次打印步骤制造的过氧化氢传感器。尽管灵敏度有所降低，但由于噪声大大降低，导致所得的整体改性传感器的线性校准范围（5×10-1×10 M）更宽，检测限约为表面改性传感器的四分之一，平均而言，信号噪声比提高了六倍。相应的葡萄糖和乳酸生物传感器与基于表面改性换能器的生物传感器相比，表现出相似甚至更高的灵敏度。通过对人血清的分析验证了这些生物传感器。与传统的表面改性传感器相比，生产单次打印步骤整体改性传感器所需的时间和成本减少，以及其分析性能特点有望使其在（生物）传感器领域得到广泛应用。
