Chemistry Faculty of M.V. Lomonosov, Moscow State University, 119991 Moscow, Russia.
Sensors (Basel). 2023 Apr 15;23(8):4014. doi: 10.3390/s23084014.
We report new enzyme-containing siloxane membranes for biosensor elaboration. Lactate oxidase immobilization from water-organic mixtures with a high concentration of organic solvent (90%) leads to advanced lactate biosensors. The use of the new alkoxysilane monomers-(3-aminopropyl)trimethoxysilane (APTMS) and trimethoxy[3-(methylamino)propyl]silane (MAPS)-as the base for enzyme-containing membrane construction resulted in a biosensor with up to a two times higher sensitivity (0.5 A·M·cm) compared to the biosensor based on (3-aminopropyl)triethoxysilane (APTES) we reported previously. The validity of the elaborated lactate biosensor for blood serum analysis was shown using standard human serum samples. The developed lactate biosensors were validated through analysis of human blood serum.
我们报告了新的含酶硅氧烷膜，用于生物传感器的研制。在高有机溶剂浓度（90%）的水-有机混合物中固定乳酸氧化酶，可得到先进的乳酸生物传感器。使用新的烷氧基硅烷单体-（3-氨基丙基）三甲氧基硅烷（APTMS）和三甲氧基[3-（甲基氨基）丙基]硅烷（MAPS）作为酶含量膜构建的基础，与我们之前报道的基于（3-氨基丙基）三乙氧基硅烷（APTES）的生物传感器相比，生物传感器的灵敏度提高了近两倍（0.5 A·M·cm）。使用标准人血清样本证明了所研制的乳酸生物传感器在血清分析中的有效性。通过分析人血血清验证了所开发的乳酸生物传感器。
