Fu Xiaomei, Tan Yan, Shi Meng, Zeng Chaoxi, Qin Si
College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China.
Foods. 2023 Feb 10;12(4):773. doi: 10.3390/foods12040773.
Critical flavonoids from have huge potential bioactivities on hypoglycemic. A multi-index comprehensive assessment based on Analytic Hierarchy Process (AHP) method was performed to optimize the extraction process of flavonoids from , which taking dihydromyricetin, taxifolin, myricetin and quercetin as indexes, and, then, an in vitro simulated gastrointestinal digestion model was established to investigate the changes of flavonoids contents and their antioxidant capacity before and after digestion. The results showed that three influence factors acted significantly with the order of ethanol concentration > solid-liquid ratio > ultrasound time. The optimized extraction parameters were as follows: 1:37 / of solid-liquid ratio, 68% of ethanol concentration and 45 min for ultrasonic time. During in vitro digestion, the order of remaining ratio of four flavonoids in the extract was dihydromyricetin > taxifolin > myricetin > quercetin in gastric digestion, and remaining ratio of taxifolin was 34.87% while others were restructured in intestinal digestion. Furthermore, the 1,1-dipheny-2-picryhydrazyl free radical (DPPH ·) scavenging ability and oxygen radical absorption capacity (ORAC) of extract were more stable in gastric digestion. After an hour's intestinal digestion, the extract had no DPPH antioxidant capacity, but amazingly, its ORAC antioxidant capacity was retained or increased, which implied that substances were transformed and more hydrogen donors were produced. This study has carried out a preliminary discussion from the perspective of extraction and put forward a new research idea, to improve the in vivo bioavailability of the critical flavonoids from .
来自[具体来源未提及]的关键黄酮类化合物在降血糖方面具有巨大的潜在生物活性。基于层次分析法（AHP）进行了多指标综合评估，以优化从[具体来源未提及]中提取黄酮类化合物的工艺，该工艺以二氢杨梅素、花旗松素、杨梅素和槲皮素为指标，然后建立体外模拟胃肠道消化模型，研究消化前后黄酮类化合物含量及其抗氧化能力的变化。结果表明，三个影响因素作用显著，顺序为乙醇浓度＞固液比＞超声时间。优化后的提取参数如下：固液比1:37（g/mL），乙醇浓度68%，超声时间45分钟。在体外消化过程中，提取物中四种黄酮类化合物的残留率顺序在胃消化中为二氢杨梅素＞花旗松素＞杨梅素＞槲皮素，在肠消化中花旗松素的残留率为34.87%，而其他成分发生了重构。此外，提取物的1,1-二苯基-2-苦基肼自由基（DPPH·）清除能力和氧自由基吸收能力（ORAC）在胃消化中更稳定。经过一小时的肠消化后，提取物没有DPPH抗氧化能力，但令人惊讶的是，其ORAC抗氧化能力得以保留或增强，这意味着发生了物质转化并产生了更多的氢供体。本研究从提取角度进行了初步探讨，提出了新的研究思路，以提高来自[具体来源未提及]的关键黄酮类化合物的体内生物利用度。
