Department of Food Science, Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, 400372 Cluj-Napoca, Romania.
Food Investigation and Innovation Group, Food Technology Department, Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain.
Nutrients. 2022 Aug 26;14(17):3512. doi: 10.3390/nu14173512.
Brewers' spent grain (BSG), the main by-product of the brewing industry, is a rich source of minerals and water-soluble vitamins such as thiamine, pyridoxine, niacin, and cobalamin. Bioaccessibility through digestion is an important step toward the complete absorption of minerals and B group vitamins in the gastrointestinal system. Inductively coupled plasma optical emission spectrometry (ICP-OES) together with inductively coupled plasma quadrupole mass spectrometry (ICP-MS) was used for the quantification of the macro- and micro-minerals. An ultra-high performance liquid chromatography (UHPLC) system coupled with a diode array detector (DAD) was used for B group vitamin identification. Four different industrial BSG samples were used in the present study, with different percentages of malted cereals such as barley, wheat, and degermed corn. Calcium's bioaccessibility was higher in the BSG4 sample composed of 50% malted barley and 50% malted wheat (16.03%), while iron presented the highest bioaccessibility value in the BSG2 sample (30.03%) composed of 65% Pale Ale malt and 35% Vienna malt. On the other hand, vitamin B1 had the highest bioaccessibility value (72.45%) in the BSG3 sample, whilst B6 registered the lowest bioaccessibility value (16.47%) in the BSG2 sample. Therefore, measuring the bioaccessibilty of bioactive BSG compounds before their further use is crucial in assessing their bioavailability.
啤酒糟（BSG）是酿造工业的主要副产品，是矿物质和水溶性维生素（如硫胺素、吡哆醇、烟酸和钴胺素）的丰富来源。通过消化实现生物利用度是矿物质和 B 族维生素在胃肠道系统中完全吸收的重要步骤。电感耦合等离子体发射光谱法（ICP-OES）和电感耦合等离子体质谱法（ICP-MS）用于测定常量和微量元素。超高效液相色谱（UHPLC）系统与二极管阵列检测器（DAD）结合用于 B 族维生素的鉴定。本研究使用了四种不同的工业 BSG 样品，其中含有不同比例的麦芽谷物，如大麦、小麦和去胚玉米。由 50%麦芽大麦和 50%麦芽小麦组成的 BSG4 样品中钙的生物利用度较高（16.03%），而由 65% Pale Ale 麦芽和 35% Vienna 麦芽组成的 BSG2 样品中铁的生物利用度最高（30.03%）。另一方面，BSG3 样品中维生素 B1 的生物利用度最高（72.45%），而 BSG2 样品中 B6 的生物利用度最低（16.47%）。因此，在进一步使用之前测量 BSG 化合物的生物利用度对于评估其生物利用度至关重要。
