Wu Lei, Jin Xiang, Zheng Canwen, Ma Fengjing, Zhang Xue, Gao Pengfei, Gao Jianhua, Zhang Liwei
Institute of Molecular Science, Shanxi University, Taiyuan 030006, China.
Shanxi Key Laboratory of Minor Crops Germplasm Innovation and Molecular Breeding, College of Life Sciences, Shanxi Agricultural University, Jinzhong 030801, China.
Foods. 2023 Feb 16;12(4):854. doi: 10.3390/foods12040854.
Mao Jian Tea (MJT) has been generally consumed as a digestive aid for more than a hundred years in the Shanxi province of China. However, determination of its efficacy still remains elusive. This study investigated the effect of Mao Jian Green Tea (MJGT) on gastrointestinal motility. The biphasic effects of the hydro extracts of MJGT on gastric emptying and small intestinal propulsion of rats were identified in vivo; namely, the low (MJGT_L) and medium (MJGT_M) concentrations promoted gastrointestinal motility ( < 0.05), whereas the high concentration (MJGT_H) showed the opposite effect ( < 0.01). The expression levels of the gastric hormones, GAS, MTL and VIP ( < 0.05) were consistent with the gastrointestinal motility variation, with the exception of MTL in MJGT_H group ( > 0.01). Two flavonoids, eriodictyol (0.152 mg/mL) and luteolin (0.034 mg/mL), and the corresponding glycosides eriodictyol-7--glucoside (0.637 mg/mL) and luteolin-7--glucoside (0.216 mg/mL), dominated the hydro extracts identified by HPLC and UPLC-ESI-MS. These compounds can regulate the muscle strip contractions isolated from the gastrointestinal tissues. Additionally, the different concentrations also influenced the gut microbiota accordingly characterized by 16S rDNA gene sequencing. The MJGT_L boosted several probiotic bacteria, such as Muribaculaceae (1.77-fold), Prevotellaceae (1.85-fold) and Lactobacillaceae (2.47-fold), and suppressed the pathogenic species such as Staphylococcaceae (0.03-fold) that, conversely, was enriched in the MJGT_H group (1.92-fold). Therefore, the biphasic effect indicated that the dosage of the herbal tea should not be overlooked.
在中国山西省，毛尖茶（MJT）作为助消化饮品已被广泛饮用了一百多年。然而，其功效仍不明确。本研究调查了毛尖绿茶（MJGT）对胃肠动力的影响。在体内实验中确定了MJGT水提取物对大鼠胃排空和小肠推进的双相作用；即低浓度（MJGT_L）和中浓度（MJGT_M）促进胃肠动力（P<0.05），而高浓度（MJGT_H）则显示相反作用（P<0.01）。胃激素GAS、MTL和VIP的表达水平（P<0.05）与胃肠动力变化一致，但MJGT_H组的MTL除外（P>0.01）。通过HPLC和UPLC-ESI-MS鉴定，水提取物中主要含有两种黄酮类化合物，圣草酚（0.152mg/mL）和木犀草素（0.034mg/mL），以及相应的糖苷圣草酚-7-O-葡萄糖苷（0.637mg/mL）和木犀草素-7-O-葡萄糖苷（0.216mg/mL）。这些化合物可调节从胃肠组织分离的肌条收缩。此外，不同浓度还相应地影响了肠道微生物群，通过16S rDNA基因测序进行表征。MJGT_L增加了几种益生菌，如毛螺菌科（1.77倍）、普雷沃氏菌科（1.85倍）和乳杆菌科（2.47倍），并抑制了葡萄球菌科等病原菌（0.03倍），相反，葡萄球菌科在MJGT_H组中富集（1.92倍）。因此，这种双相作用表明凉茶的剂量不容忽视。
