Key Laboratory for Forest Genetic and Tree Improvement &Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming 650224, China.
Key Laboratory of State Forestry Administration on Biodiversity Conservation in Southwest China, Southwest Forestry University, Kunming 650224, China.
Genes (Basel). 2023 Jan 21;14(2):278. doi: 10.3390/genes14020278.
(1) Background: Lignin is a unique component of the secondary cell wall, which provides structural support for perennial woody plants. are the core factors of the auxin-signaling pathway, which plays an important role in promoting plant growth, but the specific relationship between auxin response factors and lignin has not been fully elucidated with regard to rapid plant growth in forest trees. (2) Objectives: This study aimed to investigate the relationship between and lignin with regard to rapid plant growth in forest trees. (3) Methods: We used bioinformatics analysis to investigate the family, find genes homologous to and in , and explore the changes in gene expression and lignin content under light treatment. (4) Results: We identified and characterized 35 based on chromosome-level genome data from . In total, we identified 92 genes in , , and , which were subsequently divided into three subgroups based on phylogenetic analysis and classified the conserved exon-intron structures and motif compositions of the into the same subgroups. Collinearity analysis suggested that segmental duplication and whole-genome duplication events were majorly responsible for the expansion of the family, and the analysis of Ka/Ks indicated that the majority of the duplicated underwent purifying selection. The analysis of cis-acting elements showed that were sensitive to light, plant hormones, and stress. We analyzed the tissue-specific transcription profiles of with transcriptional activation function and the transcription profiles of with high expression under light in the stem. We also measured the lignin content under light treatment. The data showed that the lignin content was lower, and the gene transcription profiles were more limited under red light than under white light on days 1, 7, and 14 of the light treatments. The results suggest that may be involved in the regulation of lignin synthesis, thereby promoting the rapid growth of . (5) Conclusions: Collectively, this study firstly reports that may be involved in the regulation of lignin synthesis and in promoting the rapid growth in
（1）背景：木质素是次生细胞壁的独特成分，为多年生木本植物提供结构支撑。Auxin response factors（ARFs）是生长素信号通路的核心因子，在促进植物生长中发挥着重要作用，但在林木快速生长中，Auxin response factors（ARFs）与木质素之间的具体关系尚未完全阐明。（2）目的：本研究旨在探讨Auxin response factors（ARFs）与木质素在林木快速生长中的关系。（3）方法：我们使用生物信息学分析方法，研究了 家族，发现了与 和 在 中同源的基因，并探讨了光照处理下基因表达和木质素含量的变化。（4）结果：我们基于 的染色体水平基因组数据鉴定并描述了 35 个 。总共在 、 和 中鉴定了 92 个 基因，随后根据系统发育分析将其分为三个亚组，并将 的保守外显子-内含子结构和基序组成进行了分类归入同一亚组。共线性分析表明，片段复制和全基因组复制事件是 家族扩张的主要原因，Ka/Ks 分析表明，大多数复制的 经历了纯化选择。顺式作用元件分析表明， 对光照、植物激素和胁迫敏感。我们分析了具有转录激活功能的 的组织特异性转录谱和在茎中高表达的 在光照下的转录谱。我们还测量了光照处理下的木质素含量。结果表明，在光照处理的第 1、7 和 14 天，红光下木质素含量较低，基因转录谱也较窄。这表明 可能参与木质素合成的调节，从而促进 的快速生长。（5）结论：总之，本研究首次报道 可能参与木质素合成的调节，并促进
