Inhibits Lipid Deposition in Goat Intramuscular Preadipocytes.

Malonyl-CoA decarboxylase () is a major regulator of fatty acid oxidation catalyzing the decarboxylation of malonyl coenzyme A (malonyl-CoA). Although its involvement in human diseases has been well studied, its role in intramuscular fat (IMF) deposition remains unknown. In this present study, 1726 bp of cDNA was cloned (OM937122) from goat liver, including 5'UTR of 27 bp, 3'UTR of 199 bp, and CDS of 1500 bp, encoding 499 amino acids. In this present study, although the overexpression of increased the mRNA expression of and , the expression of and was also activated significantly and resulted in a decrease in cellular lipid deposition in goat intramuscular preadipocytes. Meanwhile, the silencing of increased the cellular lipid deposition and was accompanied by the expression activation of and the expression suppression of and , despite the expression suppression of genes related to fatty acid synthesis, including and . However, the expression of was not affected significantly ( > 0.05) by the expression alteration of in this present study. Furthermore, 2025 bp of promoter was obtained and predicted to be regulated by C/EBPα, SP1, SREBP1, and PPARG. In summary, although different pathways may respond to the expression alteration of , the expression of was negatively correlated with the cellular lipid deposition in goat intramuscular preadipocytes. These data may be beneficial for enhancing our understanding of the regulation of IMF deposition in goats.