Faculty of Medicine, University of Cologne, 50923 Cologne, Germany.
Department of Neurology, University Hospital Cologne, 50931 Cologne, Germany.
Genes (Basel). 2023 Feb 9;14(2):454. doi: 10.3390/genes14020454.
Stroke patients show some degree of spontaneous functional recovery, but this is not sufficient to prevent long-term disability. One promising approach is to characterize the dynamics of stroke recovery genes in the lesion and distant areas. We induced sensorimotor cortex lesions in adult C57BL/6J mice using photothrombosis and performed qPCR on selected brain areas at 14, 28, and 56 days post-stroke (P14-56). Based on the grid walk and rotating beam test, the mice were classified into two groups. The expression of cAMP pathway genes , , and , was higher in poor- compared to well-recovered mice in contralesional primary motor cortex (cl-MOp) at P14&56 and cl-thalamus (cl-TH), but lower in cl-striatum (cl-Str) at P14 and cl-primary somatosensory cortex (cl-SSp) at P28. Plasticity and axonal sprouting genes, and , were decreased in cl-MOp at P14 and cl-Str at P28 and increased in cl-SSp at P28 and cl-Str at P14, respectively. In the cl-TH, was increased, and decreased at P14. , also involved in axonal sprouting, was only increased in poor-recovered mice in cl-MOp at P28. The results underline the gene expression dynamics and spatial variability and challenge existing theories of restricted neural plasticity.
中风患者表现出一定程度的自发性功能恢复，但这不足以防止长期残疾。一种有前途的方法是描述病变和远处区域中风恢复基因的动力学。我们使用光血栓形成在成年 C57BL/6J 小鼠中诱导感觉运动皮层损伤，并在中风后 14、28 和 56 天（P14-56）对选定的大脑区域进行 qPCR。根据网格行走和旋转梁测试，将小鼠分为两组。在 P14 和 P28 的 cl-Str 和 P14 的 cl-SSp 中，在 P14 和 P56 的 cl-MOp 和 cl-TH 中，与恢复良好的小鼠相比，cAMP 通路基因 、 和 在对侧运动皮层（cl-MOp）和对侧丘脑（cl-TH）中的表达更高，但在 P14 的 cl-Str 和 P28 的 cl-SSp 中表达较低。可塑性和轴突发芽基因 、 和 在 P14 的 cl-MOp 和 P28 的 cl-Str 中降低，在 P28 的 cl-SSp 和 P14 的 cl-Str 中增加。在 cl-TH 中， 在 P14 时增加， 在 P14 时减少。 ，也参与轴突发芽，仅在 P28 的 cl-MOp 中恢复不佳的小鼠中增加。这些结果强调了基因表达的动态变化和空间变异性，挑战了现有的神经可塑性受限理论。
