Department of Zoology, Ghazi University, Dera Ghazi Khan 32200, Pakistan.
Institute of Zoology, Bahauddin Zakariya University, Multan 60800, Pakistan.
Genes (Basel). 2023 Feb 10;14(2):455. doi: 10.3390/genes14020455.
Chronic periodontal disease (CP) is a multifactorial infectious and inflammatory disease that occurs due to the challenge between the immune response of the host and specific periodontal bacteria, and that can lead to tooth loss due to damage inflicted to the supporting tissue. The current study investigates the genotypes of the and genes, along with the allelic frequency of the single nucleotide polymorphism [SNP; rs1695] in the GSTP1 gene and correlates them individually or in various combinations with the incidence of CP.
A total of 203 clinically confirmed CP patients and 201 control subjects were enrolled from Multan and Dera Ghazi Khan Districts in Pakistan from April to July 2022. Multiplex Polymerase Chain Reaction (PCR) and tetra-primer amplification refractory mutation system-polymerase chain reaction (T-ARMS-PCR) approaches were applied to determine the genotypes of the studied GSTs. The association of rs1695 in with CP was studied both individually and in various combinations with and .
The absence of , the presence of and the presence of the mutant allele (G) at rs1695 in were found to be significantly associated with CP. Patients aged between 10 and 30 years were more affected by CP.
Our results indicate that the genotypes of the analyzed GSTs affect the levels of protection from oxidative stress and may therefore influence the disease progression in CP.
慢性牙周病（CP）是一种多因素的感染性和炎症性疾病，由于宿主的免疫反应与特定牙周细菌之间的相互作用而发生，并可能因对支持组织造成的损伤而导致牙齿丧失。本研究调查了 和 基因的基因型，以及 GSTP1 基因中单核苷酸多态性 [SNP; rs1695]的等位基因频率，并将它们单独或组合与 CP 的发生相关联。
2022 年 4 月至 7 月，从巴基斯坦木尔坦和德拉加齐汗地区招募了 203 名经临床确诊的 CP 患者和 201 名对照。应用多重聚合酶链反应（PCR）和四引物扩增受阻突变系统-聚合酶链反应（T-ARMS-PCR）方法确定所研究 GST 的基因型。单独和与 和 组合研究 rs1695 在 中的作用与 CP 的关系。
发现缺失 ，存在 和 rs1695 中的突变等位基因（G）与 CP 显著相关。年龄在 10 至 30 岁之间的患者更容易受到 CP 的影响。
我们的结果表明，分析的 GST 基因型影响对氧化应激的保护水平，因此可能影响 CP 的疾病进展。
