Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, College of Horticulture, Hainan University, Haikou 570228, China.
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Genes (Basel). 2023 Feb 13;14(2):476. doi: 10.3390/genes14020476.
Cabbage ( var. ) is a vegetable rich in glucosinolates (GSLs) that have proven health benefits. To gain insights into the synthesis of GSLs in cabbage, we systematically analyzed GSLs biosynthetic genes (GBGs) in the entire cabbage genome. In total, 193 cabbage GBGs were identified, which were homologous to 106 GBGs in . Most GBGs in cabbage have undergone negative selection. Many homologous GBGs in cabbage and Chinese cabbage differed in expression patterns indicating the unique functions of these homologous GBGs. Spraying five exogenous hormones significantly altered expression levels of GBGs in cabbage. For example, MeJA significantly upregulated side chain extension genes and and the expression of core structure construction genes and , while ETH significantly repressed the expression of side chain extension genes such as , and , and some transcription factors, namely , , , and . Phylogenetically, the family and and subfamilies may only be involved in GSL synthesis in cruciferous plants. Our unprecedented identification and analysis of GBGs in cabbage at the genome-wide level lays a foundation for the regulation of GSLs synthesis through gene editing and overexpression.
甘蓝（变种）是一种富含硫代葡萄糖苷（GSLs）的蔬菜，已被证明对健康有益。为了深入了解甘蓝中 GSL 的合成，我们系统地分析了整个甘蓝基因组中的 GSL 生物合成基因（GBGs）。共鉴定出 193 个甘蓝 GBGs，与. 中的 106 个 GBGs 同源。甘蓝中的大多数 GBGs 经历了负选择。甘蓝和白菜中许多同源的 GBGs 在表达模式上存在差异，表明这些同源的 GBGs 具有独特的功能。喷洒五种外源激素显著改变了甘蓝中 GBGs 的表达水平。例如，MeJA 显著上调侧链延伸基因 和 以及核心结构构建基因 和 的表达，而 ETH 显著抑制侧链延伸基因 和 的表达，如 、 和 ，以及一些转录因子，即 、 、 和 。系统发育分析表明， 家族和 和 亚家族可能仅参与十字花科植物的 GSL 合成。我们在全基因组水平上对甘蓝 GBGs 的空前鉴定和分析为通过基因编辑和过表达来调控 GSLs 合成奠定了基础。
