Department of Biotechnology, University of Sargodha, Sargodha 40100, Pakistan.
Diabetes and Cardio-Metabolic Disorders Lab, Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Jhang Road, Faisalabad 38000, Pakistan.
Genes (Basel). 2023 Feb 17;14(2):515. doi: 10.3390/genes14020515.
The intake of various types and amounts of dietary fats influences metabolic and cardiovascular health. Hence, this study evaluated the impact of routinely consumed Pakistani dietary fats on their cardiometabolic impact. For this, we made four groups of mice, each comprising 5 animals: (1) C-ND: Control mice on a normal diet, (2) HFD-DG: High-fat diet mice on a normal diet plus 10% (/) desi ghee, (3) HFD-O: Mice on normal diet plus 10% (/) plant oil (4) HFD-BG: Mice on normal diet plus 10% (/) banaspati ghee. Mice were fed for 16 weeks, and blood, liver, and heart samples were collected for biochemical, histological, and electron microscopic analysis. The physical factors indicated that mice fed on HFD gained more body weight than the C-ND group. Blood parameters do not show significant differences, but overall, the glucose and cholesterol concentrations were raised in the mice fed with a fat-rich diet, with the highest concentrations in the HFD-BG group. The mice fed with HFD-BG and HFD-O had more lipid droplets in the liver, compared to HFD-DG and C-ND.
各种类型和数量的膳食脂肪的摄入会影响代谢和心血管健康。因此，本研究评估了巴基斯坦日常饮食中常用脂肪对其心脏代谢影响。为此，我们将 5 只老鼠分为 4 组：（1）C-ND：正常饮食的对照老鼠；（2）HFD-DG：正常饮食加 10%（/）印度酥油的高脂肪饮食老鼠；（3）HFD-O：正常饮食加 10%（/）植物油的老鼠；（4）HFD-BG：正常饮食加 10%（/）巴纳萨蒂酥油的老鼠。老鼠喂食 16 周后，收集血液、肝脏和心脏样本进行生化、组织学和电子显微镜分析。生理因素表明，喂食高脂肪饮食的老鼠比 C-ND 组体重增加更多。血液参数没有显示出显著差异，但总的来说，喂食富含脂肪的饮食会导致老鼠的血糖和胆固醇浓度升高，其中 HFD-BG 组的浓度最高。与 HFD-DG 和 C-ND 相比，喂食 HFD-BG 和 HFD-O 的老鼠肝脏中有更多的脂质滴。
