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phi112 的特征分析，一个与玉米基因紧密连锁的分子标记，及其在区分普通玉米和 QPM 的多重 PCR 中的应用。

Characterization of phi112, a Molecular Marker Tightly Linked to the Gene of Maize, and Its Utilization in Multiplex PCR for Differentiating Normal Maize from QPM.

机构信息

ICAR-Indian Institute of Maize Research, P.A.U. Campus, Ludhiana 141004, India.

ICAR-Indian Institute of Maize Research, Pusa Campus, Delhi 110012, India.

出版信息

Genes (Basel). 2023 Feb 20;14(2):531. doi: 10.3390/genes14020531.

DOI:10.3390/genes14020531
PMID:36833458
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9957476/
Abstract

Quality Protein Maize (QPM) contains higher amounts of essential amino acids lysine and tryptophan. The QPM phenotype is based on regulating zein protein synthesis by transcription factor. Many gene modifiers act to optimize the amino acid content and agronomic performance. An SSR marker, phi112, is present upstream of the DNA gene. Its analysis has shown the presence of transcription factor activity. The functional associations of opaque2 have been determined. The putative transcription factor binding at phi112 marked DNA was identified through computational analysis. The present study is a step towards understanding the intricate network of molecular interactions that fine-tune the QPM genotype to influence maize protein quality. In addition, a multiplex PCR assay for differentiation of QPM from normal maize is shown, which can be used for Quality Control at various stages of the QPM value chain.

摘要

优质蛋白玉米（QPM）含有更高量的必需氨基酸赖氨酸和色氨酸。QPM 表型基于通过转录因子调节玉米醇溶蛋白的合成。许多基因修饰因子作用于优化氨基酸含量和农艺性能。SSR 标记 phi112 存在于 DNA 基因的上游。其分析表明存在转录因子活性。已经确定了 opaque2 的功能关联。通过计算分析鉴定了在 phi112 标记 DNA 处的假定转录因子结合。本研究是朝着理解精细调节 QPM 基因型以影响玉米蛋白质质量的分子相互作用复杂网络迈出的一步。此外，还展示了用于区分 QPM 和普通玉米的多重 PCR 检测，可用于 QPM 价值链的各个阶段的质量控制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/100b/9957476/1eed91c98816/genes-14-00531-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/100b/9957476/182da3babacb/genes-14-00531-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/100b/9957476/b99778c6a3bf/genes-14-00531-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/100b/9957476/46cc91ebc236/genes-14-00531-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/100b/9957476/13a3c5e81351/genes-14-00531-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/100b/9957476/1eed91c98816/genes-14-00531-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/100b/9957476/182da3babacb/genes-14-00531-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/100b/9957476/b99778c6a3bf/genes-14-00531-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/100b/9957476/46cc91ebc236/genes-14-00531-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/100b/9957476/13a3c5e81351/genes-14-00531-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/100b/9957476/1eed91c98816/genes-14-00531-g005.jpg

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本文引用的文献

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Rapid prediction of winter wheat yield and nitrogen use efficiency using consumer-grade unmanned aerial vehicles multispectral imagery.利用消费级无人机多光谱影像快速预测冬小麦产量和氮素利用效率
Front Plant Sci. 2022 Oct 24;13:1032170. doi: 10.3389/fpls.2022.1032170. eCollection 2022.
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Expression profile of protein fractions in the developing kernel of normal, Opaque-2 and quality protein maize.
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Temporal profiling of essential amino acids in developing maize kernel of normal, - and QPM germplasm.正常、opaque-2和优质蛋白玉米种质发育中玉米籽粒必需氨基酸的时间特征分析
Physiol Mol Biol Plants. 2020 Feb;26(2):341-351. doi: 10.1007/s12298-019-00724-x. Epub 2019 Dec 4.
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Long-read sequencing reveals genomic structural variations that underlie creation of quality protein maize.长读测序揭示了导致优质蛋白玉米产生的基因组结构变异。
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