Liubarev A E, Kurganov B I
Mol Biol (Mosk). 1987 Sep-Oct;21(5):1286-96.
In virtue of analysis of data on the interaction of tricarboxylic acid cycle enzymes with the mitochondrial inner membrane and data on the enzyme-enzyme interactions, the spatial structure for the tricarboxylic acid cycle enzyme complex (tricarboxylic acid cycle metabolon) is proposed. The alpha-ketoglutarate dehydrogenase complex, adsorbed on the mitochondrial inner membrane along one of its 3-fold symmetry axes, plays the key role in the formation of metabolon. Two association sites of the alpha-ketoglutarate dehydrogenase complex located on opposite sides of the complex participate in the interaction with the membrane. The tricarboxylic acid cycle enzyme complex contains one molecule of the alpha-ketoglutarate dehydrogenase complex and six molecules of each of the other enzymes of the tricarboxylic acid cycle, as well as aspartate aminotransferase and nucleosidediphosphate kinase. Succinate dehydrogenase, the integral protein of the mitochondrial inner membrane, is a component of the anchor site responsible for the assembly of metabolon on the membrane. The molecular mass of the complex (ignoring succinate dehydrogenase) is of 8.10(6) daltons. The metabolon symmetry corresponds to the D3 point symmetry group. It is supposed, that the tricarboxylic acid cycle enzyme complex interacts with other multienzyme complexes of the matrix and the electron transfer chain.
通过对三羧酸循环酶与线粒体内膜相互作用的数据以及酶 - 酶相互作用的数据进行分析，提出了三羧酸循环酶复合物（三羧酸循环代谢体）的空间结构。沿其三重对称轴之一吸附在线粒体内膜上的α - 酮戊二酸脱氢酶复合物在代谢体的形成中起关键作用。位于复合物相对两侧的α - 酮戊二酸脱氢酶复合物的两个缔合位点参与与膜的相互作用。三羧酸循环酶复合物包含一分子α - 酮戊二酸脱氢酶复合物以及三羧酸循环其他每种酶的六个分子，还有天冬氨酸氨基转移酶和核苷二磷酸激酶。琥珀酸脱氢酶是线粒体内膜的整合蛋白，是负责在膜上组装代谢体的锚定位点的一个组成部分。该复合物（忽略琥珀酸脱氢酶）的分子量为8.10(6)道尔顿。代谢体的对称性对应于D3点对称群。据推测，三羧酸循环酶复合物与基质和电子传递链的其他多酶复合物相互作用。
