Toxicological Research Laboratory-Lapetox, University of Sorocaba, Sorocaba 18023-000, SP, Brazil.
Center for Natural and Human Sciences, Federal University of ABC, Santo André 09210-580, SP, Brazil.
Int J Environ Res Public Health. 2023 Feb 10;20(4):3114. doi: 10.3390/ijerph20043114.
(Pb) is a toxic metal, responsible for several damages to human health. (Ab) is a mushroom with promising antioxidant properties to be used as an alternative chelator in Pb intoxication. The aim was to understand the Pb toxicokinetic and the potential of Ab as a protective agent. A total of 20 female Wistar rats were distributed into 4 groups (n = 5/group): Control (receiving water); Group Ab 100 mg/kg (gavage); Group Pb 100 mg/L in water; and Group Ab + Pb-100 mg/kg + 100 mg/L (gavage and water). Pb administration occurred daily until the 19th day of pregnancy. On day 19 of gestation, the rats were euthanized, and the blood and tissues were collected for Pb measurement, using an inductively coupled plasma mass spectrometer. The results showed that the levels of Pb in the blood, placenta, and liver of the mothers, and in the brain of the fetuses increased significantly in the Pb group. On the other hand, the combined exposure to Pb + Ab showed a significant decrease in the metal concentration in relation to the Pb group, returning to normal levels. Kidney and bone lead levels also increased significantly in the Pb group. However, in the combined exposure group, levels did not return to the control amounts; there was protection, but the Pb concentration was still significantly higher than in the control. In the brain, no significant differences were observed. In conclusion, we suggest is a natural chelator, because the co-administration of the mushroom was able to interact with Pb ions, minimizing the Pb absorption and distribution. These effects are suggested since have antioxidants and beta glucan that interact with Pb, chelating it and, thus, reducing its toxic effects.
（Pb）是一种有毒金属，对人类健康造成多种损害。（Ab）是一种具有良好抗氧化特性的蘑菇，可用作 Pb 中毒的替代螯合剂。目的是了解 Pb 的毒代动力学和 Ab 作为保护剂的潜力。总共将 20 只雌性 Wistar 大鼠分为 4 组（n = 5/组）：对照组（给予水）；Ab 组 100mg/kg（灌胃）；Pb 组 100mg/L 水；和 Ab + Pb-100mg/kg+100mg/L 组（灌胃和水）。Pb 给药每天进行，直到妊娠第 19 天。妊娠第 19 天，处死大鼠，采集血液和组织，用电感耦合等离子体质谱仪测量 Pb 含量。结果表明，母亲血液、胎盘和肝脏以及胎儿大脑中的 Pb 水平在 Pb 组中显著升高。另一方面，与 Pb 组相比，联合暴露于 Pb + Ab 导致金属浓度显著降低，恢复正常水平。Pb 组的肾脏和骨骼中的 Pb 水平也显著升高。然而，在联合暴露组中，水平没有恢复到对照水平；有保护作用，但 Pb 浓度仍然明显高于对照。在大脑中，没有观察到显著差异。总之，我们认为 是一种天然螯合剂，因为蘑菇的共同给药能够与 Pb 离子相互作用，最大限度地减少 Pb 的吸收和分布。这些作用是由于 具有抗氧化剂和 β 葡聚糖与 Pb 相互作用，螯合它，从而降低其毒性作用。
