National Institute of Food Science and Technology, University of Agriculture, Faisalabad, Pakistan.
Institute of Physiology and Pharmacology, University of Agriculture, Faisalabad, Pakistan.
J Food Biochem. 2021 Dec;45(12):e13979. doi: 10.1111/jfbc.13979. Epub 2021 Oct 26.
The current research work was designed to investigate the protective effects of white button mushroom (Agaricus bisporus) against 7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancer. Breast cancer was induced in rats by the administration of a single dose of 50 mg/kg DMBA via gavage. The rats were divided into four groups: G1 (negative control group), G2 (positive control group), G3 (rats receiving mushroom extract), and G4 (rats administered with doxorubicin). The mushroom extract significantly (p < .001) improved the activity of antioxidant enzymes in carcinogenic rats. Moreover, the mushroom extract also prevented the increase in the concentration of tumor biomarkers that are CEA, CA 15.3, and CRP in experimental rats. Liver function enzymes were also raised in G2 and G4 compared with G3. Besides, the RBCs and Hb were also reduced significantly in G4 while in G3. The mushroom extract effectively controlled the level of RBCs and Hb. An improvement in lipid profile was also measured in mushroom extract receiving rats. Conclusively, the mushroom extract alleviated DMBA-induced breast cancer potentially via improving antioxidants, reducing lipid peroxidation, and decreasing tumor biomarkers. PRACTICAL APPLICATIONS: The present research study examined the antitumor potential of white button mushroom. The mushroom effectively prevented the increase in tumor biomarkers, reduction in antioxidant enzymes, and increase in lipid peroxidation in rats with DMBA-induced breast cancer. The mushroom can be used as a potential source to prevent breast cancer and further research can be conducted to explore its anticancer mechanisms.
当前的研究工作旨在研究白蘑菇（双孢蘑菇）对 7,12-二甲基苯并[a]蒽（DMBA）诱导的乳腺癌的保护作用。通过灌胃向大鼠给予 50mg/kg DMBA 的单剂量来诱导乳腺癌。将大鼠分为四组：G1（阴性对照组）、G2（阳性对照组）、G3（接受蘑菇提取物的大鼠）和 G4（给予多柔比星的大鼠）。蘑菇提取物显著（p<0.001）提高了致癌大鼠抗氧化酶的活性。此外，蘑菇提取物还防止了实验大鼠中肿瘤标志物 CEA、CA 15.3 和 CRP 浓度的增加。与 G3 相比，G2 和 G4 中的肝功能酶也升高。此外，G4 中的 RBC 和 Hb 也显著降低，而 G3 中的 RBC 和 Hb 则降低。蘑菇提取物有效控制 RBC 和 Hb 的水平。在接受蘑菇提取物的大鼠中，还测量到脂质谱的改善。总之，蘑菇提取物通过改善抗氧化剂、降低脂质过氧化和减少肿瘤标志物，有效地控制了 DMBA 诱导的乳腺癌。
本研究考察了白蘑菇的抗肿瘤潜力。蘑菇有效防止了 DMBA 诱导的乳腺癌大鼠中肿瘤标志物的增加、抗氧化酶的减少和脂质过氧化的增加。蘑菇可以用作预防乳腺癌的潜在来源，可以进一步研究其抗癌机制。
