Quaye Emmanuel Kofi, Adjei Raymond Lovelace, Isawumi Abiola, Allen David J, Caporaso J Gregory, Quaye Osbourne
West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), Department of Biochemistry, Cell and Molecular Biology, University of Ghana, Accra P.O. Box LG 54, Ghana.
Council for Scientific and Industrial Research (CSIR)-Animal Research Institute, Accra P.O. Box AH 20, Ghana.
Int J Mol Sci. 2023 Feb 10;24(4):3607. doi: 10.3390/ijms24043607.
Acute gastroenteritis (AGE) is a disease of global public health importance. Recent studies show that children with AGE have an altered gut microbiota relative to non-AGE controls. Yet, how the gut microbiota differs in Ghanaian children with and without AGE remains unclear. Here, we explore the 16S rRNA gene-based faecal microbiota profiles of Ghanaian children five years of age and younger, comprising 57 AGE cases and 50 healthy controls. We found that AGE cases were associated with lower microbial diversity and altered microbial sequence profiles relative to the controls. The faecal microbiota of AGE cases was enriched for disease-associated bacterial genera, including , and . In contrast, the faecal microbiota of controls was enriched for potentially beneficial genera, including , , , and . Lastly, distinct microbial correlation network characteristics were observed between AGE cases and controls, thereby supporting broad differences in faecal microbiota structure. Altogether, we show that the faecal microbiota of Ghanaian children with AGE differ from controls and are enriched for bacterial genera increasingly associated with diseases.
急性肠胃炎（AGE）是一种具有全球公共卫生重要性的疾病。最近的研究表明，与非急性肠胃炎对照组相比，患有急性肠胃炎的儿童肠道微生物群发生了改变。然而，在加纳，患急性肠胃炎和未患急性肠胃炎的儿童的肠道微生物群有何不同仍不清楚。在此，我们探索了5岁及以下加纳儿童基于16S rRNA基因的粪便微生物群谱，其中包括57例急性肠胃炎病例和50名健康对照。我们发现，与对照组相比，急性肠胃炎病例的微生物多样性较低，微生物序列谱也有所改变。急性肠胃炎病例的粪便微生物群中与疾病相关的细菌属增多，包括 、 和 。相比之下，对照组的粪便微生物群中潜在有益菌属增多，包括 、 、 和 。最后，在急性肠胃炎病例和对照组之间观察到了不同的微生物相关网络特征，从而支持了粪便微生物群结构的广泛差异。总之，我们表明，加纳患急性肠胃炎儿童的粪便微生物群与对照组不同，且与疾病相关的细菌属增多。
