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真细菌中类前病毒基因的基因组分布及 、 、 中 CpdB 样蛋白的酶特异性比较

Genomic Distribution of Pro-Virulent -like Genes in Eubacteria and Comparison of the Enzyme Specificity of CpdB-like Proteins from , and .

机构信息

Grupo de Enzimología, Departamento de Bioquímica y Biología Molecular y Genética, Facultad de Medicina y Ciencias de la Salud, Universidad de Extremadura, 06006 Badajoz, Spain.

Unidad de Aparato Digestivo, Hospital de Zafra, Área de Salud Llerena-Zafra, Servicio Extremeño de Salud, 06300 Zafra, Spain.

出版信息

Int J Mol Sci. 2023 Feb 19;24(4):4150. doi: 10.3390/ijms24044150.

DOI:10.3390/ijms24044150
PMID:36835561
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9958556/
Abstract

The gene is pro-virulent in avian pathogenic and in , where it encodes a periplasmic protein named CpdB. It is structurally related to cell wall-anchored proteins, CdnP and SntA, encoded by the also pro-virulent and genes of and , respectively. CdnP and SntA effects are due to extrabacterial hydrolysis of cyclic-di-AMP, and to complement action interference. The mechanism of CpdB pro-virulence is unknown, although the protein from non-pathogenic hydrolyzes cyclic dinucleotides. Considering that the pro-virulence of streptococcal CpdB-like proteins is mediated by c-di-AMP hydrolysis, CpdB activity was tested as a phosphohydrolase of 3'-nucleotides, 2',3'-cyclic mononucleotides, linear and cyclic dinucleotides, and cyclic tetra- and hexanucleotides. The results help to understand pro-virulence in and are compared with CpdB and SntA, including the activity of the latter on cyclic-tetra- and hexanucleotides reported here for the first time. On the other hand, since CpdB-like proteins are relevant to host-pathogen interactions, the presence of -like genes was probed in eubacterial taxa by TblastN analysis. The non-homogeneous genomic distribution revealed taxa with -like genes present or absent, identifying eubacteria and plasmids where they can be relevant.

摘要

该基因在禽致病性 和 中具有原毒力，在这些细菌中，它编码一种名为 CpdB 的周质蛋白。它与细胞壁锚定蛋白 CdnP 和 SntA 在结构上相关，这两种蛋白分别由也具有原毒力的 和 基因编码。CdnP 和 SntA 的作用是由于细菌外切环二核苷酸 AMP 的水解，以及互补作用干扰。CpdB 原毒力的机制尚不清楚，尽管来自非致病性 的蛋白水解环二核苷酸。考虑到链球菌 CpdB 样蛋白的原毒力是由 c-di-AMP 水解介导的，因此测试了 CpdB 作为 3'-核苷酸、2'、3'-环单核苷酸、线性和环二核苷酸以及环四核苷酸和六核苷酸的磷酸水解酶的活性。这些结果有助于了解 在 中的原毒力，并与 CpdB 和 SntA 进行了比较，包括这里首次报道的后者对环四核苷酸和六核苷酸的活性。另一方面，由于 CpdB 样蛋白与宿主-病原体相互作用有关，因此通过 TblastN 分析探测了真核生物分类单元中的 -样基因。非均匀的基因组分布揭示了存在或不存在 -样基因的分类单元，确定了它们可能相关的真核生物和质粒。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35c5/9958556/1f9b13a4462f/ijms-24-04150-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35c5/9958556/a386f9e2f051/ijms-24-04150-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35c5/9958556/ef177d75fa98/ijms-24-04150-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35c5/9958556/591052504a85/ijms-24-04150-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35c5/9958556/5f166b9341a0/ijms-24-04150-sch002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35c5/9958556/9c5d179e3790/ijms-24-04150-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35c5/9958556/1f9b13a4462f/ijms-24-04150-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35c5/9958556/a386f9e2f051/ijms-24-04150-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35c5/9958556/ef177d75fa98/ijms-24-04150-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35c5/9958556/591052504a85/ijms-24-04150-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35c5/9958556/5f166b9341a0/ijms-24-04150-sch002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35c5/9958556/9c5d179e3790/ijms-24-04150-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35c5/9958556/1f9b13a4462f/ijms-24-04150-g004.jpg

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