Grupo de Enzimología, Departamento de Bioquímica y Biología Molecular y Genética, Facultad de Medicina y Ciencias de la Salud, Universidad de Extremadura, 06006 Badajoz, Spain.
Genes (Basel). 2023 Aug 20;14(8):1657. doi: 10.3390/genes14081657.
UshA and CpdB are nucleotidases of the periplasm of several Gram-negative bacteria, while several Gram-positives contain cell wall-bound variants. UshA is a 5'-nucleotidase, a UDP-sugar hydrolase, and a CDP-alcohol hydrolase. CpdB acts as a 3'-nucleotidase and as a phosphodiesterase of 2',3'-cyclic nucleotides and 3',5'-linear and cyclic dinucleotides. Both proteins are pro-virulent for the pathogens producing them and facilitate escape from the innate immunity of the infected host. Recently, the genomic distribution of -like genes in was found to be non-homogeneous among different taxa, and differences occur within single taxa, even at species level. Similitudes and differences between UshA-like and CpdB-like proteins prompted parallel analysis of their genomic distributions in . The presence of -like and -like genes was tested by TBlastN analysis using seven protein probes to query the NCBI Complete Genomes Database. It is concluded that the distribution of -like genes, like that of -like genes, is non-homogeneous. There is a partial correlation between both gene kinds: in some taxa, both are present or absent, while in others, only one is present. The result is an extensive catalog of the genomic distribution of these genes at different levels, from phylum to species, constituting a starting point for research using other in silico or experimental approaches.
UshA 和 CpdB 是几种革兰氏阴性菌周质中的核苷酸酶，而几种革兰氏阳性菌含有细胞壁结合的变体。UshA 是一种 5'-核苷酸酶、UDP-糖水解酶和 CDP-醇水解酶。CpdB 作为 3'-核苷酸酶和 2'、3'-环核苷酸以及 3'、5'-线性和环状二核苷酸的磷酸二酯酶起作用。这两种蛋白都对产生它们的病原体具有前毒性，并有助于逃避受感染宿主的先天免疫。最近，在不同分类群中发现了-like 基因在基因组中的分布不均匀，并且在单个分类群中甚至在物种水平上也存在差异。UshA 样和 CpdB 样蛋白之间的相似性和差异促使对它们在基因组中的分布进行平行分析。使用 7 种蛋白质探针进行 TBlastN 分析来测试-like 和 -like 基因的存在，以查询 NCBI 完整基因组数据库。得出的结论是-like 基因的分布与-like 基因的分布不均匀。这两种基因之间存在部分相关性：在一些分类群中，两者都存在或不存在，而在其他分类群中，只有一种存在。这是这些基因在不同水平（从门到种）的基因组分布的广泛目录，为使用其他计算机或实验方法进行研究提供了起点。
